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New research on cord blood from Jagiellonian University summarized
November 26th, 2007
2007 NOV 26 -- According to recent research from Krakow, Poland, "To determine whether monocytes can be generated from CD34(+) hematopoietic progenitors in large numbers, cord blood CD34(+) cells were first expanded for 3-10 days in X-VIVO 10 medium supplemented with FCS, stem cell factor (SCF), thrombopoietin (TPO), and Flt-3 Ligand (Flt-3L), and then differentiated in IMDM medium supplemented with FCS, SCF, Flt-3L, IL-3 and MCSF for 7-14 days. These two step cultures resulted in up to a 600-fold mean increase of total CD14(+) cells." "Using this approach, two subpopulations of monocytes were obtained: CD14(+)CD16(-) and CD14(++)CD16(+) occurring at 2:1 ratio. 1.25(OH)(2) Vitamin D3 added to the differentiation medium altered this ratio by decreasing proportion of CD14(++)CD16(+) monocytes. In comparison to CD14(+)CD16(-), the CD14(++)CD16(+) cells showed different morphology and an enhanced expression of CD11b, CD33, CD40, CD64, CD86, CD163, HLA-DR, and CCR5. Both subpopulations secreted TNF and IL-12p40 but little or no IL-10. CD14(++)CD16(+) monocytes released significantly more IL-12p40, were better stimulators of MLR but showed less S. aureus phagocytosis," wrote M. Stec and colleagues, Jagiellonian University. The researchers concluded: "These subpopulations are clearly different from those present in the blood and may be novel monocyte subsets that represent different stages in monocyte differentiation with distinct biological function." Stec and colleagues published their study in the Journal of Leukocyte Biology (Expansion and differentiation of CD14(+) CD16 and CD14(++) CD16(+) human monocyte subsets from cord blood CD34(+) hematopoietic progenitors. Journal of Leukocyte Biology, 2007;82(3):594-602). For additional information, contact M. Zembala, Jagiellonian University, College Medical, Polish amer Institute Pediatrics, Dept. of Clinic Immunology & Transplantation, Wielicka Str 265, PL-30663 Krakow, Poland. Publisher contact information for the Journal of Leukocyte Biology is: Federation American Society Experimental Biology, 9650 Rockville Pike, Bethesda, MD 20814-3998, USA. Keywords: Poland, Krakow, Blood Transfusion, Cord Blood, Medical Device, Stem Cell Research, Transfusion Medicine, Jagiellonian University. This article was prepared by Hematology Week editors from staff and other reports. Copyright 2007, Hematology Week via NewsRx.com.
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