Data on enzyme research published by M. Sole and colleagues
December 1st, 2008
2008 DEC 1 -- "We investigated the influence of potential laccase inducers with environmental relevance on extracellular laccase activity and removal of the xenoestrogen technical nonylphenol (tNP) by the aquatic hyphomycete Clavariopsis aquatica. Concomitantly, we identified two putative laccase gene fragments (lcc1 and lcc2) and have followed their expression during removal of tNP under different conditions," researchers in Leipzig, Germany report.
"Our results indicate a significant effect of copper on extracellular laccase activity in supernatants of fungal cultures. Laccase activity was highest in the presence of copper when added together with vanillic acid, followed by copper when used alone. Only slight laccase activities were recorded in the presence of only vanillic acid, whereas in the absence of either compound laccase activities were negligible. Laccase activity was well correlated with the removal efficiency of tNP, indicating the involvement of laccase in tNP bioconversion. Overall, lcc2 was less expressed than lcc1," wrote M. Sole and colleagues.
The researchers concluded: "The expression of lcc1 and lcc2 correlated only partially with the measured laccase activity, suggesting the existence of cell-associated laccase fractions not detectable in fungal culture supernatants and/or the existence of additional laccase genes."
Sole and colleagues published their study in Fems Microbiology Letters (Extracellular laccase activity and transcript levels of putative laccase genes during removal of the xenoestrogen technical nonylphenol by the aquatic hyphomycete Clavariopsis aquatica. Fems Microbiology Letters, 2008;288(1):47-54).
For additional information, contact M. Sole, UFZ, Helmholtz Center Environmental Research, Dept. of Environmental Microbiology, Permoserstr 15, D-04318 Leipzig, Germany.
Publisher contact information for the journal Fems Microbiology Letters is: Blackwell Publishing, 9600 Garsington Rd., Oxford OX4 2DQ, Oxon, England.
