Reports summarize ductal carcinoma in situ research from Shandong University
December 15th, 2008
2008 DEC 15 -- " WWOX and FHIT are two candidate tumor suppressor genes located inactive fragile sites, the damage of which has been associated with the development of breast cancer. The association of the expression of these genes and the development of breast cancer has not been fully explored," scientists in Jinan, People's Republic of China report.
"We evaluated mRNA and protein expression of WWOX and FHIT in breast tissue with normal histological appearances, atypical ductal hyperplasia, ductal carcinoma in situ, and invasive cancer to see if a progressive decline in expression was present. Reverse transcription-polymerase chain reaction and Western blotting were used to evaluate the specimens for mRNA and protein expression, including 28 specimens with normal tissue, 28 specimens with atypical ductal hyperplasia, 33 specimens with ductal carcinoma in situ, and 51 specimens with invasive ductal carcinoma. Compared with in situ and invasive cancer specimens, both normal and atypical hyperplasia specimens had greater rates of detectable mRNA (WWOX rate ratio=2.95, 95% Cl 1.24-7.08; FHIT rate ratio=4.58, 95% Cl 1.82-11.81) and Western blotting detectable protein (WWOX rate ratio=4.12, 95% Cl 1.63-10.73; FHIT rate ratio=3.76, 95% Cl 1.44-10.06). For both proteins, differences between normal and atypical hyperplasia specimens and between in situ and invasive carcinoma specimens were explainable by chance (P >0.05 for each analysis). Within each histological category, differences among fractions of specimens showed that FHIT and WWOX mRNA and protein expression were explainable by chance (P >0.05 for each analysis)," wrote T.T. Wang and colleagues, Shandong University.
The researchers concluded: " Expression of FHIT and WWOX decreases along with breast tissue progress from a normal histological appearance to atypical ductal hyperplasia, in situ cancer, and the final invasive cancer."
Wang and colleagues published their study in Chinese Medical Journal (Loss expression of active fragile sites genes associated with the severity of breast epithelial abnormalities. Chinese Medical Journal, 2008;121(20):1969-1974).
For additional information, contact S.Y. Hu, Shandong University, Qilu Hospital, Dept. of General Surgery, Jinan 250012, Shandong, People's Republic of China.
The publisher's contact information for the Chinese Medical Journal is: Chinese Medical Association, 42 Dongsi Xidajie, Beijing 100710, People's Republic of China.
Keywords: People's Republic of China, Jinan, Biotechnology, Breast Cancer, Breast Carcinoma, Breast Neoplasm, Diagnosis, Diagnostics, Ductal Carcinoma in Situ, Enzyme Research, Gene Therapy, Genetics, Genomics, Hyperplasia, Neoplasms, Oncology, Polymerase, Protein Expression, Proteins, Proteomics, Tumor Suppression, Women's Health, Shandong University.
This article was prepared by Clinical Oncology Week editors from staff and other reports. Copyright 2008, Clinical Oncology Week via NewsRx.com.
