New DNA libraries research reported from L. Chen and co-authors

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March 9th, 2009

   2009 MAR 9 -- "To identify potential tumor antigen by immunoscreening the urinary bladder cancer cDNA library with monoclonal antibodies. Monoclonal antibodies were prepared," scientists in Xian, People's Republic of China report.

   "A cDNA expression library was constructed from bladder cancer cell line BLZ211. Immunogenic proteins were identified by immunoscreening the cDNA library with ten monoclonal antibodies. The cDNA library of BLZ211 cells was established using lambda ZAP as vector. The titer of unamplified cDNA library was 1.39x10(6) pfu/ml with a recombinant rate of 97.72%, and titer of amplified one was 8.4x10(9) pfu/ml. After immunoscreening, ten positive clones representing ten different antigens were identified, which include two proteins with unknown function; coactosin-like 1, eukaryotic translation elongation factor, HNRPA1, histidine triad nucleotide binding protein, KRT7, LCN2, TSTA3, zinc finger protein, C11orf48 and HSPC148. The cDNA library was of high quality and can be used in further study," wrote L. Chen and colleagues.

   The researchers concluded: "By immunoscreening the bladder cancer cDNA library with ten monoclonal antibodies, we identified ten immunogenic proteins that otherwise would not have been identified as potential diagnostic marker and vaccinogens of bladder cancer using the gene discovery effort."

   Chen and colleagues published their study in World Journal of Urology (Immunoscreening of urinary bladder cancer cDNA library and identification of potential tumor antigen. World Journal of Urology, 2009;27(1):107-112).

   For additional information, contact X. Li, Xi An Jiao Tong University, Affiliated Hospital 1, School Medical, Center Clinic Molecular Biology, Xian 710061, People's Republic of China.

   The publisher's contact information for the World Journal of Urology is: Springer, 233 Spring St., New York, NY 10013, USA.

   Keywords: People's Republic of China, Xian, Biotechnology, Bladder Cancer, Bladder Carcinoma, DNA Libraries, Genomics, Monoclonal Antibody, Oncology, Proteomics, Urology.

   This article was prepared by Biotech Business Week editors from staff and other reports. Copyright 2009, Biotech Business Week via NewsRx.com.

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