New leukemia gene therapy data have been reported by S. Kaneko and co-authors
March 16th, 2009
2009 MAR 16 -- "Long-term clinical remissions of leukemia, after allogeneic hematopoietic stem cell transplantation, depend on alloreactive memory T cells able to self-renew and differentiate into antileukemia effectors. This is counterbalanced by detrimental graft-versus-host disease (GVHD)," investigators in Milan, Italy report.
"Induction of a selective suicide in donor T cells is a current gene therapy approach to abrogate GVHD. Unfortunately, genetic modification reduces alloreactivity of lymphocytes. This associates with an effector memory (T-EM) phenotype of gene-modified lymphocytes and may limit antileukemia effect. We hypothesized that alloreactivity of gene-modified lymphocytes segregates with the central memory (T-CM) phenotype. To this, we generated suicide gene-modified T-CM lymphocytes with a retroviral vector after CD28 costimulation and culture with IL-2, IL-7, or a combination of IL-7 and IL-15. In vitro, suicide gene-modified T-CM cells self-renewed upon alloantigen stimulation and resisted activation-induced cell death. In a humanized mouse model, only suicide gene -modified T cells cultured with IL-7 and IL-15 persisted, differentiated in T-EM cells, and were as potent as unmanipulated lymphocytes in causing GVHD. GVHD was halted through the activation of the suicide gene machinery," wrote S. Kaneko and colleagues.
The researchers concluded: "These results warrant the use of suicide gene modified T-CM cells cultured with IL-7 and IL-15 for the safe exploitation of the alloreactive response against cancer. (Blood. 2009;113:1006-1015)'."
Kaneko and colleagues published their study in Blood (IL-7 and IL-15 allow the generation of suicide gene-modified alloreactive self-renewing central memory human T lymphocytes. Blood, 2009;113(5):1006-1015).
For additional information, contact A. Bondanza, Ist Science San Raffaele, Cancer Immunotherapy & Gene Therapy Program, Experimental Hematology Unit, Dept. of Oncology, Via Olgettina 58, I-20132 Milan, Italy.
The publisher of the journal Blood can be contacted at: American Society Hematology, 1900 M Street. NW Suite 200, Washington, DC 20036, USA.
