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Research results from Amgen, Inc. update understanding of spectroscopy
2009 NOV 2 -- "We evaluated the effect of UV-B light at 302 nm on a model therapeuticprotein, 2.6 D type I soluble tumor necrosis factor receptor (sTNF-RI). This protein contains a single Trp at position 97 and seven native disulfide bonds along its interior from the N to the C-terminus," investigators in the United States report.
"At a protein concentration of 0.1 mg/mL photoirradiation was found to induce the formation of soluble disulfide cross-linked dimers with greater levels of these species formed at pH 8 than at pH 5. Intermolecular disulfide formation was also directly correlated with the photoinduced unfolding of the protein as measured by changes in...
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