Medical News Article on Biotechnology

Studies from Zurich University of Applied Sciences Further Understanding of Biotechnology

2012 FEB 21 -- "Pure methanol, which is required as an inducer of the AOX1 promoter and a carbon/energy source in processes for recombinant protein production by Pichia pastoris, is impracticable and therefore generally undesirable. As an alternative, a procedure using double carbon substrate was examined (11.7 g(carbon) l(-1), 60%/40% carbon from glucose/methanol)," researchers in Wadenswil, Switzerland report.

"The effects on methanol metabolism, extracellular formation of porcine trypsinogen, biomass growth and cell viability were analyzed. In contrast to batch cultures, where the glucose and methanol were utilized sequentially, in carbon/energy-limited...

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