Research on rectal cancer discussed by A. Hopuncheung and co-researchers
2009 JUL 14 - (NewsRx.com) -- According to a study from Montpellier, France, "Gene expression quantification using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) requires data normalization using an invariable reference gene. Here we assessed the stability of 15 housekeeping genes in 31 tumor and normal rectal samples to validate a reliable reference gene for rectal cancer studies." "Our data show that 18S and 28S RNA are stably expressed in all samples. Moreover, when used for normalization, 18S, but not 28S. greatly reduced unspecific variations of gene expression due to RNA degradation," wrote A. Hopuncheung and colleagues. The researchers concluded: "These results demonstrate that 18S is an appropriate reference gene for normalization of RT-qPCR data from rectal cancer samples." Hopuncheung and colleagues published their study in Analytical Biochemistry (Validation of an appropriate reference gene for normalization of reverse transcription-quantitative polymerase chain reaction data from rectal cancer biopsies. Analytical Biochemistry, 2009;388(2):348-350). For more information, contact A. Hopuncheung, Val dAurelle Cancer Institute, Dept. of Biology, F-34298 Montpellier, France. Publisher contact information for the journal Analytical Biochemistry is: Academic Press Inc. Elsevier Science, 525 B St., Ste. 1900, San Diego, CA 92101-4495, USA. Keywords: France, Montpellier, Biochemistry, Diagnosis, Diagnostics, Enzyme Research, Oncology, Polymerase, Rectal Cancer, Rectal Carcinoma. This article was prepared by Cancer Weekly editors from staff and other reports. Copyright 2009, Cancer Weekly via NewsRx.com.
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