Gene Therapy Weekly

Studies in the area of colon cancer reported from Qingdao University

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"In this study, for the first time we constructed recombinant adenovirus to investigate the inhibitory effects of RhoA and RhoC shRNAs in tandem expression on the cell proliferation and invasion of colorectal cancer HCT116 cells. The recombinant adenovirus carrying RhoA and RhoC shRNAs in tandem expression was transfected into HCT116. The mRNA transcription and protein expressions of RhoA and RhoC were examined by RT-FQPCR and Western blot respectively. Cellular proliferation inhibitory activity was determined by methyl thiazolyl tetrazolium (MTT) assay and invasive and migrating potential was detected through in vitro Matrigel coated invasion and migration assay. Both mRNA and proteins Levels of RhoA and RhoC were significantly reduced in HCT116 cells transfected with Ad-A1+A2+C1+C2 than those in Ad-HK group and control one. The relative RhoA and RhoC mRNA transcriptions were decreased to 40% and 36% (P < 0.05), while proteins expression reducing 42% and 35%, respectively (P < 0.05). Growth curves analysis showed that alive cell number in the Ad-A1+A2+C1+C2 group was lower than others in the third to sixth day and transwell chamber analysis showed that migration/invasion activity was significantly suppressed in Ad-A1+A2+C1+C2 group. Our results indicate recombinant adenovirus carrying RhoA and RhoC shRNAs in tandem expression may inhibit the growth and invasion of HCT116 cells," wrote X.P. Liu and colleagues, Qingdao University.

The researchers concluded: "Application of such vector to inhibit one or more genes may be a new method to cancer therapy."

Liu and colleagues published their study in the Journal of Experimental & Clinical Cancer Research (Inhibitory effects of adenovirus mediated tandem expression of RhoA and RhoC shRNAs in HCT116 cells. Journal of Experimental & Clinical Cancer Research, 2009;28():52).

For additional information, contact H.B. Wang, Qingdao University, College Medical, Affiliated Hospital, Dept. of General Surgery, Qingdao 266003, People's Republic of China.

Publisher contact information for the Journal of Experimental & Clinical Cancer Research is: Biomedical Central Ltd., Current Science Group, Middlesex House, 34-42 Cleveland St., London W1T 4LB, England.

Keywords: People's Republic of China, Qingdao, Adenoviridae, Biotechnology, Cancer Research, Carcinogenesis, Cell Proliferation, Colon Cancer, Colon Carcinoma, Colorectal, Gastroenterology, Gene Therapy, Oncology, Protein Expression, Proteins, Proteomics, Qingdao University.

This article was prepared by Gene Therapy Weekly editors from staff and other reports. Copyright 2009, Gene Therapy Weekly via NewsRx.com.