New gene therapy findings from Y.H. Lu and co-researchers published
2008 JAN 17 -- According to recent research published in the journal Applied Microbiology and Biotechnology, "To seek more information on function of two component regulatory systems (TCSs) in Streptomyces coelicolor, a dozen TCS-knockout mutants were generated, and phenotype changes were determined. One TCS (SCO5403/5404)-deleted mutant with phenotype change was obtained." "Here, we report the characterization of this novel TCS, designated as RapA1/A2 (regulation of both actinorhodin and a type I polyketide), using genetic and proteomic approaches. Although growth and morphological analyses showed no difference between the knockout mutant and wild-type strain M145, a visible decrease of the production of actinorhodin (Act) was observed in rapA1/A2 mutant. The decrease can be restored by introducing rapA1/ A2 genes on an integrative vector. A 2D-gel based proteomic analysis showed that knockout of rapA1/ A2 resulted in reduced expression of a putative 3-oxoacyl-[acyl-carrier protein] reductase that is part of a biosynthetic cluster for a cryptic type I polyketide. Further reverse-transcriptase polymerase chain reaction (RT-PCR) analyses confirmed that expression levels of several biosynthetic genes and the respective pathway-specific regulatory genes actII-ORF4 and kasO for these two clusters were all down-regulated in the rapA1/A2 mutant, compared to M145," wrote Y.H. Lu and colleagues. The researchers concluded: "Taken together, the results demonstrated that RapA1/A2 may serve as a positive regulator for biosynthesis of both Act and the uncharacterized polyketide in S. coelicolor, and the effects exerted by RapA1/A2 were dependent on the pathway specific regulatory genes." Lu and colleagues published their study in Applied Microbiology and Biotechnology (Characterization of a novel two-component regulatory system involved in the regulation of both actinorhodin and a type I polyketide in Streptomyces coelicolor. Applied Microbiology and Biotechnology, 2007;77(3):625-635). For additional information, contact W.H. Jiang, Chinese Academy Science, Shanghai Institute Biology Science, Institute Plant Physiol & Ecology, Molecular Microbiology Laboratory, 345 Lingling Lu, Shanghai 200032, People's Republic of China. The publisher's contact information for the journal Applied Microbiology and Biotechnology is: Springer, 233 Spring Street, New York, NY 10013, USA. Keywords: People's Republic of China, Shanghai, Applied Microbiology, Biotechnology, Gene Therapy, Genetics, Genomics, Metabolism, Proteomics. This article was prepared by Gene Therapy Weekly editors from staff and other reports. Copyright 2008, Gene Therapy Weekly via NewsRx.com.
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