Researchers from University of South Dakota report recent findings in cervical cancer
2008 JAN 18 -- According to recent research from the United States, "Cervical cancer is the leading gynecological malignancy worldwide, and the incidence of this disease is very high in American Indian women. Infection with the human papillomavirus (HPV) is responsible for more than 95% of cervical squamous carcinomas." "Therefore, the main objective of this study was to analyze oncogenic HPV infections in American Indian women residing in the Northern Plains. Cervical samples were collected from 287 women attending a Northern Plains American Indian reservation outpatient clinic. DNA was extracted from the cervical samples and HPV-specific DNA was amplified by polymerase chain reaction (PCR) using the L1 consensus primer sets. The PCR products were hybridized with the Roche HPV Line Blot assay for HPV genotyping to detect 27 different low- and high-risk HPV genotypes. The Chi-squared test was performed for statistical analysis of the HPV infection and cytology diagnosis data. Of the total 287 patients, 61 women (21.25%) tested positive for HPV infection. Among all HPV-positive women, 41 (67.2%) were infected with high-risk HPV types. Of the HPV infected women, 41% presented with multiple HPV genotypes. Additionally, of the women infected with oncogenic HPV types, 20 (48.7%) were infected with HPV16 and 18 and the remaining 21 (51.3%) were infected with other oncogenic types (i.e., HPV59, 39, 73). Women infected with oncogenic HPV types had significantly higher (p=0.001) abnormal Papanicolaou smear tests (Pap test) compared to women who were either HPV negative or positive for non-oncogenic HPV types. The incidence of HPV infection was inversely correlated (p < 0.05) with the age of the patients, but there was no correlation (p=0.33) with seasonal variation. In this study, we observed a high prevalence of HPV infection in American Indian women residing on Northern Plains Reservations," wrote M.C. Bell and colleagues, University of South Dakota. The researchers concluded: "In addition, a significant proportion of the oncogenic HPV infections were other than HPV16 and 18." Bell and colleagues published their study in Gynecologic Oncology (There is a high prevalence of human papillomavirus infection in American Indian women of the Northern Plains. Gynecologic Oncology, 2007;107(2):236-241). For additional information, contact S.C. Chauhan, University of South Dakota, Cancer Biology Research Institute, Sanford Research, 1400 W 22nd St., Sioux Falls, SD, USA. Publisher contact information for the journal Gynecologic Oncology is: Academic Press Inc. Elsevier Science, 525 B St., Ste. 1900, San Diego, CA 92101-4495, USA. Keywords: United States, Sioux Falls, Cancer Diagnosis, Carcinoma Diagnosis, Cervical Cancer, Cervical Carcinoma, Cytology, DNA, Diagnosis, Diagnostics, Enzyme Research, Genotyping, Gynecology, HPV, Human Papillomavirus, Oncology, Papillomavirus Infections, Polymerase, Squamous Cell Carcinoma, Women's Health, University of South Dakota. This article was prepared by Genomics & Genetics Weekly editors from staff and other reports. Copyright 2008, Genomics & Genetics Weekly via NewsRx.com.
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