New findings reported from Kyung-Hee University describe advances in hepatitis A virus
2009 JUL 13 - (NewsRx.com) -- According to a study from South Korea, "We describe the secretory expression and immunogenicity of the recombinant HAV (hepatitis A virus) structural polypeptide VPI from stably transformed Drosophila melanogaster S2 (Schneider 2) cells. Southern-blot analysis indicated that transformed S2 cells contained multiple copies of the HAV VPI gene in the genome." "Recombinant VPI was secreted into a culture medium with a molecular mass of 42-49 kDa. A maximum production level of 6.24 mg of recombinant VPI/litre was obtained in a T-flask culture of Drosophila S2 cells S days after induction with 0.5 mM CuSO4. The recombinant HAV VPI protein elicited the production of specific IgA in the small intestine by oral immunization and production of specific IgG in the serum by intraperitoneal immunization," wrote J.M. Lee and colleagues, Kyung-Hee University. The researchers concluded: "Our findings show that secretory recombinant VPI from transformed Drosophila S2 cells can be used as an effective experimental immunogen for research in vaccine development." Lee and colleagues published the results of their research in Biotechnology and Applied Biochemistry (Expression and immunogenicity of recombinant polypeptide VPI of human hepatitis A virus in stably transformed fruitfly (Drosophila melanogaster) Schneider 2 cells. Biotechnology and Applied Biochemistry, 2009;53(Part 2):101-109). For additional information, contact I.S. Chung, Kyung Hee University, Dept. of Genetics Engineering, Suwon 449701, South Korea. The publisher of the journal Biotechnology and Applied Biochemistry can be contacted at: Portland Press Ltd., Third Floor, Eagle House, 16 Procter Street, London WC1V 6 Nx, England. Keywords: South Korea, Applied Biochemistry, Biotechnology, Gastroenterology, HAV, Hepatitis A Virus, Hepatology, Immunization, Infectious Disease, Viral, Virology, Kyung-Hee University. This article was prepared by Hepatitis Weekly editors from staff and other reports. Copyright 2009, Hepatitis Weekly via NewsRx.com.
|
