Research from D. Baksh and co-researchers in the area of bone research published
2008 JAN 14 -- According to recent research from Toronto, Canada, "We demonstrate that adult human bone marrow (BM) contains a population of mesenchymal stromal cells (MSCs) that can be expanded in non-adherent, cytokine-dependent, suspension culture conditions for at least 42 days. The cells generated during suspension culture lacked detectable levels of gene expression associated with differentiated mesenchymal cell types, including bone, muscle and fat, suggesting that suspension culture maintains MSCs in an uncommitted state." "However, when these undifferentiated cells were taken out of suspension culture and placed in adherent osteogenic conditions, osteogenic genes were upregulated and morphologically identifiable bone matrix was elaborate. Flow cytometric analysis of uncultured, density gradient-separated human BM revealed that colony forming unit-fibroblast (CFU-F) and CFU-osteoblast (CFU-O) activity was associated with a CD45CD49e(low) phenotype. Importantly, suspension-grown MSCS, capable of CFU-F and CFU-O development, maintained the CD45(-)CD49e(low) phenotype whereas MSCs directly cultured under adherent conditions rapidly upregulated CD49e expression and were associated with a CD45-CD49e(high) phenotype. Tracking the CD49e(low) expression under suspension culture conditions provides a mechanism to isolate an expanding suspension-grown MSC population with osteogenic potential," wrote D. Baksh and colleagues. The researchers concluded: "This could provide a potential strategy to isolate populations of MSCs, with functional osteogenic capacity, in a scalable and controllable culture system for therapeutic applications." Baksh and colleagues published their study in Biotechnology and Bioengineering (A non-contact suspension culture approach to the culture of osteogenic cells derived from a CD49e(Low) subpopulation of human bone marrow-derived cells. Biotechnology and Bioengineering, 2007;98(6):1195-1208). For additional information, contact J.E. Davies, Institute Biomaterials & Biomedical Engineering, Toronto, ON, Canada. Publisher contact information for the journal Biotechnology and Bioengineering is: John Wiley & Sons Inc., 111 River St., Hoboken, NJ 07030, USA. Keywords: Canada, Toronto, Bone Research, Bioengineering, Biotechnology, Bone Marrow, Stem Cell Research. This article was prepared by Stem Cell Week editors from staff and other reports. Copyright 2008, Stem Cell Week via NewsRx.com.
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