Data on pleural effusion described by P.K. Sharma et al
2009 JUL 13 - (NewsRx.com) -- According to a study from New Delhi, India, "The inadequacy of effector T-cell response in containment of tubercle bacilli is believed to result in the development of disseminated forms of tuberculosis (TB), such as miliary tuberculosis (MTB). Regulatory T cells (Treg) plausibly play a critical role in the immunopathogenesis of disseminated TB by suppression of effector immune response against Mycobacterium tuberculosis at the pathologic site(s)." "To understand the role of Treg cells in disseminated tuberculosis, we studied the frequency and function of Treg cells derived from the local disease site specimens (LDSS) of patients with TB pleural effusion and MTB as clinical models of contained and disseminated forms of disease, respectively. To (1) enumerate the frequency of Treg cells in bronchoalveolar lavage (BAL) fluid of patients with MTB and compare with that of peripheral blood, (2) study the role of Treg cells in suppression of local T-cell response, and (3) study the selective recruitment of Treg cells at the local disease site(s). Flow cytometry, reverse transcriptase polymerase chain reaction, and 3-(4,5-dimethylthythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT)-based cell proliferation assay. Frequency of Treg cells (CD4(+)CD25(+) FoxP3(+)) was significantly higher in LDSS in MTB along with higher levels of FoxP3 mRNA. Importantly, FoxP3(+) Treg cells obtained from the BAL of patients with MTB predominantly produced IL-10 and could suppress the autologous T-cell proliferation in response to M. tuberculosis antigen," wrote P.K. Sharma and colleagues. The researchers concluded: "Our results highlight the importance of Treg cells in suppression of effector immune response and their influence on bacillary dissemination, disease manifestation, and severity." Sharma and colleagues published their study in American Journal of Respiratory and Critical Care Medicine (FoxP3(+) Regulatory T Cells Suppress Effector T-Cell Function at Pathologic Site in Miliary Tuberculosis. American Journal of Respiratory and Critical Care Medicine, 2009;179(11):1061-1070). For more information, contact D.K. Mitra, All India Institute Med Science, Division Cellular Immunology, Dept. of Transplant Immunology & Immunogenet, New Delhi 110029, India. Publisher contact information for the American Journal of Respiratory and Critical Care Medicine is: American Thoracic Society, 1740 Broadway, New York, NY 10019-4374, USA. Keywords: India, New Delhi, Critical Care, Cutaneous Tuberculosis, Infectious Disease, Mycobacteria, Mycobacterium Tuberculosis, Oncology, Pleural Effusion. This article was prepared by Tuberculosis Week editors from staff and other reports. Copyright 2009, Tuberculosis Week via NewsRx.com.
|
