New encephalitis research from B.A. Thibodeaux and colleagues discussed
2009 JUL 8 - (NewsRx.com) -- "Current diagnosis of human flaviviral infections relies heavily on serological techniques such as the immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assay (MAC-ELISA). Broad application of this assay is hindered by a lack of standardized human positive-control sera that react with the wide variety of flaviviruses that can cause human disease, e. g., dengue virus (DENV), West Nile virus (WNV), yellow fever virus (YFV), Japanese encephalitis virus (JEV), and St," investigators in the United States report. "Louis encephalitis virus (SLEV). We have created a human-murine chimeric antibody combining the variable regions of the broadly flavivirus cross-reactive murine monoclonal antibody (MAb) 6B6C-1 and the constant region of human IgM to produce a standardized reagent capable of replacing human positive-control sera in a MAC-ELISA for the diagnosis of all human flaviviral infections," wrote B.A. Thibodeaux and colleagues. The researchers concluded: "The human-murine chimeric IgM antibody secreted from plasmid-transformed Sp2/0-Ag14 cells had a level of serological activity identical to that of 6B6C-1 as measured by ELISA, immunoblotting, and MAC- ELISA for multiple members of the flavivirus genus, including WNV, SLEV, YFV, DENV, and JEV.." Thibodeaux and colleagues published their study in Clinical and Vaccine Immunology (Development of a Human-Murine Chimeric Immunoglobulin M Antibody for Use in the Serological Detection of Human Flavivirus Antibodies. Clinical and Vaccine Immunology, 2009;16(5):679-685). For additional information, contact B.A. Thibodeaux, Center Diseases Control & Prevention, Arboviral Diseases Branch, Division Vector Borne Infectious Disease, US Dept. of HHS, National Center Zoonot Vector Borne & Enter Diseases, Coordina, 3150 Rampart Rd., Fort Collins, CO 80521, USA. The publisher of the journal Clinical and Vaccine Immunology can be contacted at: American Society Microbiology, 1752 N St. NW, Washington, DC 20036-2904, USA. Keywords: United States, Fort Collins, Arbovirus Encephalitis, Biotechnology, Central Nervous System Disease, Chimeric Antibodies, Dengue Fever, Encephalitis, Flavivirus, Immunization, Immunology, Infectious Disease, Japanese Encephalitis, Parasitic Disease, St. Louis Encephalitis, Vaccines, Vectors, Viral, West Nile Fever, West Nile Virus, Yellow Fever, Zoonoses. This article was prepared by Vaccine Weekly editors from staff and other reports. Copyright 2009, Vaccine Weekly via NewsRx.com.
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