Research conducted at J.R. Whiteaker and co-authors has provided new information about breast cancer
2008 JAN 17 -- According to recent research published in the Journal of Proteome Research, "Despite their potential to impact diagnosis and treatment of cancer, few protein biomarkers are in clinical use. Biomarker discovery is plagued with difficulties ranging from technological (inability to globally interrogate proteomes) to biological (genetic and environmental differences among patients and their tumors)." "We urgently need paradigms for biomarker discovery. To minimize biological variation and facilitate testing of proteomic approaches, we employed a mouse model of breast cancer. Specifically, we performed LC-MS/MS of tumor and normal mammary tissue from a conditional HER2/Neu-driven mouse model of breast cancer, identifying 6758 peptides representing >700 proteins. We developed a novel statistical approach (SASPECT) for prioritizing proteins differentially represented in LC-MS/MS datasets and identified proteins over- or under-represented in tumors. Using a combination of antibody-based approaches and multiple reaction monitoring-mass spectrometry (MRM-MS), we confirmed the overproduction of multiple proteins at the tissue level, identified fibulin-2 as a plasma biomarker, and extensively characterized osteopontin as a plasma biomarker capable of early disease detection in the mouse. Our results show that a staged pipeline employing shotgun-based comparative proteomics for biomarker discovery and multiple reaction monitoring for confirmation of biomarker candidates is capable of finding novel tissue and plasma biomarkers in a mouse model of breast cancer," wrote J.R. Whiteaker and colleagues. The researchers concluded: "Furthermore, the approach can be extended to find biomarkers relevant to human disease." Whiteaker and colleagues published their study in the Journal of Proteome Research (Integrated pipeline for mass spectrometry-based discovery and confirmation of biomarkers demonstrated in a mouse mode of breast cancer. Journal of Proteome Research, 2007;6(10):3962-3975). For additional information, contact A.G. Paulovich, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, Seattle, WA 98109, USA. The publisher's contact information for the Journal of Proteome Research is: American Chemical Society, 1155 16th St., NW, Washington, DC 20036, USA. Keywords: United States, Seattle, Biotechnology, Breast Cancer, Breast Carcinoma, Cancer Biomarkers, Carcinoma Biomarkers, Mass Spectrometry, Oncology, Proteome Research, Proteomics, Women's Health. This article was prepared by Women's Health Weekly editors from staff and other reports. Copyright 2008, Women's Health Weekly via NewsRx.com.
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