Studies from National Institute of Allergy and Infectious Disease update current data on DNA vaccines
2008 JAN 29 -- Fresh data on dna are presented in the report 'A West Nile virus DNA vaccine induces neutralizing antibody in healthy adults during a phase 1 clinical trial.' "West Nile virus (WNV) is a mosquito-borne flavivirus that can cause severe meningitis and encephalitis in infected individuals. We report the safety and immunogenicity of a WNV DNA vaccine in its first phase 1 human study," researchers in the United States report. "A single-plasmid DNA vaccine encoding the premembrane and the envelope glycoproteins of the NY99 strain of WNV was evaluated in an open-label study in 15 healthy adults. Twelve subjects completed the 3-dose vaccination schedule, and all subjects completed 32 weeks of evaluation for safety and immunogenicity. The development of a vaccine-induced immune response was assessed by enzyme-linked immunosorbant assay, neutralization assays, intracelluar cytokine staining, and enzyme-linked immunospot assay. The vaccine was safe and well tolerated, with no significant adverse events. Vaccine-induced T cell and antibody responses were detected in the majority of subjects. Neutralizing antibody to WNV was detected in all subjects who completed the 3-dose vaccination schedule, at levels shown to be protective in studies of horses, an incidental natural host for WNV. Further assessment of this DNA platform for human immunization against WNV is warranted," wrote J.E. Martin and colleagues, National Institute of Allergy and Infectious Disease. The researchers concluded: "TRIAL REGISTRATION: ClinicalTrials.gov identifier: NCT00106769 ." Martin and colleagues published their study in the Journal of Infectious Diseases (A West Nile virus DNA vaccine induces neutralizing antibody in healthy adults during a phase 1 clinical trial. Journal of Infectious Diseases, 2007;196(12):1732-40). For additional information, contact J.E. Martin, National Institutes of Health, Vaccine Research Center, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, 40 Convent Drive, Bethesda, MD 20892 USA.. Publisher contact information for the Journal of Infectious Diseases is: University Chicago Press, 1427 E 60th St., Chicago, IL 60637-2954, USA. Keywords: United States, Bethesda, Allergies, Allergy Medicine, Biotechnology, Central Nervous System Disease, Clinical Trial Research, DNA Research, DNA Vaccines, Encephalitis, Flavivirus, Immunization, Infectious Disease, Medical Device, Meningitis, Parasitic Disease, Vaccination, Vectors, Viral, West Nile Fever, West Nile Virus, Zoonoses. This article was prepared by World Disease Weekly editors from staff and other reports. Copyright 2008, World Disease Weekly via NewsRx.com.
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