Studies from University of Manchester in the area of enzyme research described
2009 JUN 8 - (NewsRx.com) -- According to a study from Manchester, the United Kingdom, "The plaque-control potential of commercially available amylase, lipase and protease was evaluated by observing their effects on coaggregation and on bacterial viability within various plaque microcosms. A quantitative coaggregation assay indicated that protease significantly inhibited the extent of coaggregation of Actinomyces naeslundii and Streptococcus oralis (P <0.05) and of Porphyromonas gingivalis and S. oralis." "Amylase significantly (P <0.05) increased the coaggregation of A. naeslundii versus Fusobacterium, nucleatum and A. naeslundii versus P. gingivalis. Concomitant challenge of constant-depth film fermenter-grown plaques with the enzymes did not result in detectable ecological perturbations (assessed by differential culture and denaturing gradient gel electrophoresis). Similar dosing and analysis of multiple Sorbarod devices did not reveal increases in bacterial dispersion which could result from disaggregation of extant plaques. A short-term hydroxyapatite colonization model was therefore used to investigate possible enzyme effects on early-stage plaque development. Whilst culture did not indicate significant reductions in adhesion or plaque accumulation, a vital visual assay revealed significantly increased aggregation frequency following enzyme exposure. In summary, although hydrolytic enzymes negatively influenced binary coaggregation, they did not cause statistically significant changes in bacterial viability within plaque microcosms," wrote R.G. Ledder and colleagues, University of Manchester. The researchers concluded: "In contrast, enzyme exposure increased aggregation within extant plaques." Ledder and colleagues published their study in the Journal of Medical Microbiology (An in vitro evaluation of hydrolytic enzymes as dental plaque control agents. Journal of Medical Microbiology, 2009;58(4):482-491). For more information, contact R.G. Ledder, University of Manchester, School Pharmacy & Pharmaceutical Science, Manchester M13 9PT, Lancs, UK. Publisher contact information for the Journal of Medical Microbiology is: Society General Microbiology, Marlborough House, Basingstoke Rd., Spencers Woods, Reading RG7 1AG, Berks, England. Keywords: United Kingdom, Manchester, Actinomycosis, Amylase, Dental Plaque, Enzyme Research, Enzymology, Fusobacterium, Lipase, Protease, University of Manchester. This article was prepared by Proteomics Weekly editors from staff and other reports. Copyright 2009, Proteomics Weekly via NewsRx.com.
|
