Research findings from U. Henssge et al update understanding of actinomycosis
2009 JUN 2 - (NewsRx.com) -- According to recent research from London, the United Kingdom, "Actinomyces naeslundii is an important early colonizer in the oral biofilm and consists of three genospecies (1, 2 and WVA 963) which cannot be readily differentiated using conventional phenotypic testing or on the basis of 16S rRNA gene sequencing. We have investigated a representative collection of type and reference strains and clinical and oral isolates (n=115) and determined the partial gene sequences of six housekeeping genes (atpA, rpoB, pgi, metG, gltA and gyrA)." "These sequences identified the three genospecies and differentiated them from Actinomyces viscosus isolated from rodents. The partial sequences of atpA and metG gave best separation of the three genospecies. A. naeslundii genospecies 1 and 2 formed two distinct clusters, well separated from both genospecies WVA 963 and A. viscosus. Analysis of the same genes in other oral Actinomyces species (Actinomyces gerencseriae, A. israelii, A. meyeri, A. odontolyticus and A. georgiae) indicated that, when sequence data were obtained, these species each exhibited <90% similarity with the A. naeslundii genospecies," wrote U. Henssge and colleagues. The researchers concluded: "Based on these data, we propose the name Actinomyces oris sp. nov. (type strain ATCC 27044(T) =CCUG 34288(T)) for A. naeslundii genospecies 2 and Actinomyces johnsonii sp. nov. (type strain ATCC 49338(T) =CCUG 3428 7(T)) for A. naeslundii genospecies WVA 963. A. naeslundii genospecies 1 should remain as A. naeslundii sensu stricto, with the type strain ATCC 12104(T) NCTC 10301(T) =CCUG 2238(T)." Henssge and colleagues published their study in International Journal of Systematic and Evolutionary Microbiology (Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp nov and Actinomyces johnsonii sp nov., previously identified as Actinomyces naeslundii genospecies 1, 2 and WVA 963. International Journal of Systematic and Evolutionary Microbiology, 2009;59(Part 3):509-516). For additional information, contact D. Beighton, Kings College London, Institute Dental, Henry Wellcome Laboratories Microbiology & Salivary Research, Dept. of Microbiology, Floor 17, Tower Wing, London SE1 9RT, UK. Publisher contact information for the International Journal of Systematic and Evolutionary Microbiology is: Society General Microbiology, Marlborough House, Basingstoke Rd., Spencers Woods, Reading RG7 1AG, Berks, England. Keywords: United Kingdom, London, Actinomycosis. This article was prepared by Life Science Weekly editors from staff and other reports. Copyright 2009, Life Science Weekly via NewsRx.com.
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