Acute Kidney Failure

Reports on ischemia findings from C. Esposito and co-researchers provide new insights

Acute Kidney Failure Library Library Home This article was published in Gastroenterology Week, which you can subscribe to online.

"In the present study we evaluated the effect of erythropoetin (EPO) in I/R injury in rats. We investigated the role of bone marrow cells (BMC) in kidney repair and the effect of EPO on BMC recruitment. Female Sprague Dawley rats transplanted with male BMCs underwent I/R injury. In the treatment group rats received 5000 IU of EPO 30 minutes before renal ischemia. At 2 and 4 weeks after I/R, rats were humanely killed and we measured creatinine clearance (glomerular filtration rate [GFR]), proteinuria, and body weight (BW). Renal tissue was harvested for histologic and molecular analysis. Fluorescein in situ hybridization (FISH) and TUNEL were used to determined the presence of male cell chimerism, and apoptosis in renal tissue. At 4 weeks after I/R, EPO significantly improved GFR (1.8 +/- 0.2 vs 1.2 +/- 0.14 mL/min; P< .05). No significant differences between EPO and control rats were observed in proteinuria, BW, and hemoglobin levels at 2 and 4 weeks. After death, the kidney showed only minimal tubulointerstitial changes, which were more marked in control rats. FISH analysis demonstrated a low degree of microchimerism, not significantly different between EPO and control rats. Apoptosis decreased between 2 and 4 weeks after I/R, in both EPO and control groups," wrote C. Esposito and colleagues.

The researchers concluded: "EPO improved GFR and injury at 4 weeks after I/R; however, it did not enhance the recruitment of BMC.."

Esposito and colleagues published their study in Transplantation Proceedings (The Improvement of Ischemia/Reperfusion Injury by Erythropoetin Is Not Mediated Through Bone Marrow Cell Recruitment In Rats. Transplantation Proceedings, 2009;41(4):1113-1115).

For more information, contact C. Esposito, Foundation IRCCS Policinico San Matteo, Unit Nephrology Dialysis & Transplantation, I-27100 Pavia, PV, Italy.

Publisher contact information for the journal Transplantation Proceedings is: Elsevier Science Inc., 360 Park Avenue South, New York, NY 10010-1710, USA.

Keywords: Italy, Pavia, Acute Kidney Failure, Acute Renal Failure, Blood Transfusion, Bone Marrow, Ischemia-Reperfusion Injury, Kidney, Kidney Transplant, Medical Device, Nephrology, Organ Transplant, Perfusion, Renal Failure, Reperfusion, Transfusion Medicine, Transplantation.

This article was prepared by Gastroenterology Week editors from staff and other reports. Copyright 2009, Gastroenterology Week via NewsRx.com.