Adenoma

Study results from University of Shizuoka provide new insights into gastroenterology

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"First published March 25, 2009; doi:10.1152/ajpcell.00638.2008. -SLC26A3, a Cl-/HCO3- exchanger, is highly expressed in intestinal epithelial cells, and its mutations cause congenital chloride diarrhea. This suggests that SLC26A3 plays a key role in NaCl absorption in the intestine. Electroneutral NaCl absorption in the intestine is mediated by functional coupling of the Na+/H+ exchanger and Cl-/HCO3- exchanger. It is proposed that the coupling of these exchangers may occur as a result of indirect linkage by changes of intracellular pH (pH(i)). We therefore investigated whether SLC26A3 is regulated by pH(i). We generated a hemagglutinin epitope-tagged human SLC26A3 construct and expressed it in Chinese hamster ovary cells. Transport activities were measured with a fluorescent chloride-sensitive dye dihydro-6-methoxy-N-ethylquinolinium iodide (diH-MEQ). pH(i) was clamped at a range of values from 6.0 to 7.4. We monitored the transport activity of SLC26A3 by reverse mode of Cl-/HCO3- and Cl-/NO3- exchange. None of these exchange modes induced membrane potential changes. At constant external pH 7.4, Cl-/HCO3- exchange was steeply inhibited with pH(i) decrease between 7.3 and 6.8 as opposed to thermodynamic prediction. In contrast, however, Cl-/NO3- exchange was essentially insensitive to pH(i) within physiological ranges. We also characterized the pH(i) dependency of COOH-terminal truncation mutants. Removal of the entire COOH-terminal resulted in decrease of the transport activity but did not noticeably affect pH(i) sensitivity. These results suggest that Cl-/HCO3- exchange mode of human SLC26A3 is controlled by a pH-sensitive intracellular modifier site, which is likely in the transmembrane domain," wrote H. Hayashi and colleagues, University of Shizuoka.

The researchers concluded: "These observations raise the possibility that SLC26A3 activity may be regulated via Na+/H+ exchanger 3 (NHE3) through the alteration of pH(i) under physiological conditions."

Hayashi and colleagues published their study in American Journal of Physiology - Cell Physiology (Regulation of intestinal Cl-/HCO3- exchanger SLC26A3 by intracellular pH. American Journal of Physiology - Cell Physiology, 2009;296(6):C1279-C1290).

For additional information, contact H. Hayashi, University of Shizuoka, Physiol Laboratory, School Nutrition Science, Suruga Ku, Yada 52-1, Shizuoka 4228526, Japan.

The publisher's contact information for the American Journal of Physiology - Cell Physiology is: American Physiological Society, 9650 Rockville Pike, Bethesda, MD 20814, USA.

Keywords: Japan, Shizuoka, Adenoma, Diarrhea, Endocrinology, Gastroenterology, Physiology, University of Shizuoka.

This article was prepared by Gastroenterology Week editors from staff and other reports. Copyright 2009, Gastroenterology Week via NewsRx.com.