Adenosine Deaminase

Research data from G.K. Yang and colleagues update understanding of gastroenterology

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"Concentrations of exogenous adenosine >= 1.0 mc M stimulated gastric release of somatostatin-like immunoreactivity (SLI), and this effect was blocked by the A(2A) receptor antagonist ZM 241385 [4-(2-[7-amino-2-(2-furyl)[1,2,4]triazolo[2,3-a][1,3,5]triazin-5-ylamino]ethyl)phenol]. The A(2A) receptor agonist CGS 21680 [2-p-(2-carboxyethyl) phenethylamino-5'-N-ethylcarboxamidoadenosine hydrochloride] augmented SLI release in a concentration-dependent manner, suggesting that A(2A) receptor activation is involved in the stimulatory effect of adenosine on SLI release. Conversely, SLI release was inhibited by the A(1) receptor agonists N-6-cyclopentyladenosine and 2-chloro-N-6-cyclopentyladenosine and lower concentration of adenosine (0.01 mc M). The involvement of specific adenosine receptors in controlling the release of gastric SLI was also examined using A(2A) receptor knockout (A(2A) R-KO) mice. In these mice, adenosine (10 mc M) inhibited SLI release, and the effect was abolished by the selective A(1) receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine, suggesting a link between the selective A(1) activation and inhibition of SLI release. The adenosine deaminase inhibitor erythro-9-(2-hydroxy-3-nonyl) adenine hydrochloride augmented SLI release in wild-type controls but not in the presence of ZM 241385 or in A(2A) R-KO mice," wrote G.K. Yang and colleagues.

The researchers concluded: "We conclude that adenosine has dual actions on regulating mouse gastric SLI release: stimulatory at higher concentrations through the A(2A) receptor and inhibitory at lower concentrations through the A(1) receptor, whereas A(2B) and A(3) receptors have a minimal role."

Yang and colleagues published their study in the Journal of Pharmacology and Experimental Therapeutics (Regulation of Somatostatin Release by Adenosine in the Mouse Stomach. Journal of Pharmacology and Experimental Therapeutics, 2009;329(2):729-737).

For additional information, contact Y.N. Kwok, DH Copp Bldg, 2146 Health Science Mall, Vancouver, BC V6T 1Z3, Canada.

Publisher contact information for the Journal of Pharmacology and Experimental Therapeutics is: American Society Pharmacology Experimental Therapeutics, 9650 Rockville Pike, Bethesda, MD 20814-3995, USA.

Keywords: Canada, Vancouver, Adenosine, Drugs, Experimental Therapeutics, Experimental Therapies, Gastric Acid Secretion, Gastroenterology, Gastrointestinal, Pharmaceuticals, Pharmacology, Therapies, Therapy, Treatment.

This article was prepared by Biotech Business Week editors from staff and other reports. Copyright 2009, Biotech Business Week via NewsRx.com.