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New adrenal cancer study findings reported from P.P. Wu and co-authors
2009 JUN 22 - (NewsRx.com) -- "(-)-Epigallocatechin-3-gallate (EGCG) is a major constituent of green tea and has been identified as an excellent anticancer agent. Nevertheless, there are no reports to date about the molecular mechanisms and signal pathways of EGCG on the induction of apoptosis in human adrenal NCI-H295 cancer cells," researchers in Taichung, Taiwan report. "The purpose of this study was to investigate the anticancer effect and molecular mechanisms of EGCG on human adrenal NCI-H295 cancer cells. The results showed that EGCG induced growth inhibition in a dose- and time-dependent manner. Moreover, it exerted low cytotoxicity on Detroit 551 normal human embryonic skin cell. When NCI-H295 cells were treated with 20 mc M EGCG, the mitochondrial membrane potential decreased and intracellular free Ca2+ increased in a time-dependent manner as analysed by flow cytometry. EGCG decreased the protein levels of Bcl-2, Bcl-xl, xIAP, clAP, Hsp70 and Hsp90, but increased the protein expression of Bad, Bax, Fas/CD95, cytochrome c, Apaf-1, AIF GADD153, GRP78, and caspase-3, -7,-8 and -9 as observed by Western blotting examination. EGCG promoted caspase-8, -9 and -3 activities in a time-dependent manner. However, pretreatment of cells with inhibitors of caspase-8, -9 and -3 led to a decrease in caspase-8, -9 and -3 activities and an increase in the percentage of viable cells," wrote P.P. Wu and colleagues. The researchers concluded: "Based on the above findings, it was confirmed that EGCG may be a drug candidate for the treatment of human adrenal cancer in the future." Wu and colleagues published their study in Anticancer Research ((-)-Epigallocatechin Gallate Induced Apoptosis in Human Adrenal Cancer NCI-H295 Cells through Caspase-dependent and Caspase-independent Pathway. Anticancer Research, 2009;29(4):1435-1442). For additional information, contact J.G. Chung, China Med University, Dept. of Biology Science & Technology, 91 Hsueh Shih Rd., Taichung 404, Taiwan. Publisher contact information for the journal Anticancer Research is: International Institute Anticancer Research, Editorial Office 1ST km Kapandritiou-Kalamou Rd. Kapandriti, PO Box 22, Athens 19014, Greece. Keywords: Taiwan, Taichung, Adrenal Cancer, Adrenal Carcinoma, Anticancer Therapy, Apoptosis, Cancer Research, Caspase, Enzyme Research, Oncology, Therapy, Treatment. This article was prepared by Biotech Business Week editors from staff and other reports. Copyright 2009, Biotech Business Week via NewsRx.com.
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