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Investigators at University of Liege Detail Research in Coenzymes

2012 DEC 25 (NewsRx) -- By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Enzymes and Coenzymes are discussed in a new report. According to news reporting from Liege, Belgium, by NewsRx journalists, research stated, "The relative contributions of the PSII-dependent and Nda2-dependent pathways for H-2 photoproduction were investigated in the green microalga Chlamydomonas reinhardtii after suphur-deprivation. For this purpose. H-2 gas production was compared for wild-type and Nda2-deficient cells with or without DCMU (a PSII-inhibitor) in the same experimental conditions."

The news correspondents obtained a quote from the research from the University of Liege, "Nda2-deficiency caused a 30% decrease of the maximal H-2 photoevolution rate observed shortly after the establishment of anoxia, and an acceleration of the decline of H-2 photoevolution rate with time. DCMU addition to Nda2-deficient cells completely inhibited H-2 photoproduction, showing that the PSII-independent H-2 photoproduction relies on the presence of Nda2, which feeds the photosynthetic electron transport chain with electrons derived from oxidative catabolism. Nda2-protein abundance increased as a result of sulphur deprivation and further during the H-2 photoproduction process, resulting in high rates of non-photochemical plastoquinone reduction in control cells. Nda2-deficiency had no significant effect on photosynthetic and respiratory capacities in sulphur-deprived cells, but caused changes in the cell energetic status (ATP and NADPH/NADP(+) ratio). The rapid decline of H-2 photoevolution rate with time in Nda2-deficient cells revealed a more pronounced inhibition of H-2 photoproduction by accumulated H-2 in the absence of non-photochemical plastoquinone reduction."

According to the news reporters, the research concluded: "Nda2 is therefore important for linking H-2 photoproduction with catabolism of storage carbon compounds, and seems also involved in regulating the redox poise of the photosynthetic electron transport chain during H-2 photoproduction."

For more information on this research see: Function of the chloroplastic NAD(P)H dehydrogenase Nda2 for H-2 photoproduction in sulphur-deprived Chlamydomonas reinhardtii. Journal of Biotechnology, 2012;162(1):81-88. Journal of Biotechnology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Biotechnology - www.elsevier.com/wps/product/cws_home/505515)

Our news journalists report that additional information may be obtained by contacting E. Mignolet, University of Liege, Inst Plant Biol B22, Lab Genet Microorganisms, B-4000 Liege, Belgium.

Keywords for this news article include: NAD, Liege, Europe, Belgium, Plastids, Chloroplasts, Dehydrogenase, Intracellular Space, Enzymes and Coenzymes, Cytoplasmic Structures

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