Research data from Purdue University update understanding of science
2007 OCT 23 -- According to recent research published in the journal Proceedings of the National Academy of Sciences of the United States of America, "'We report a chemical derivatization method that selects a class of metabolites from a complex mixture and enhances their detection by C-13 NMR. Acetylation of amines directly in aqueous medium with 1,1'-C-13(2) acetic anhydride is a simple method that creates a high sensitivity and quantitative label in complex biofluids with minimal sample pretreatment." "Detection using either 1D or 2D C-13 NMR experiments produces highly resolved spectra with improved sensitivity. Experiments to identify and compare amino acids and related metabolites in normal human urine and serum samples as well as in urine from patients with the inborn errors of metabolism tyrosinemia type 11, argininosuccinic aciduria, homocystinuria, and phenylketonuria demonstrate the method. The use of metabolite derivatization and C-13 NMR spectroscopy produces data suitable for metabolite profiling analysis of biofluids on a time scale that allows routine use. Extension of this approach to enhance the NMR detection of other classes of metabolites has also been accomplished," wrote N. Shanaiah and colleagues, Purdue University. The researchers concluded: "The improved detection of low-concentration metabolites shown here creates opportunities to improve the understanding of the biological processes and develop improved disease detection methodologies." Shanaiah and colleagues published their study in Proceedings of the National Academy of Sciences of the United States of America (Class selection of amino acid metabolites in body Fluids using chemical derivatizaflon and their enhanced C-13 NMR. Proceedings of the National Academy of Sciences of the United States of America, 2007;104(28):11540-11544). For additional information, contact D. Raftery, Purdue University, Dept. of Chemical, 560 Oval, West Lafayette, IN 47907, USA. The publisher's contact information for the journal Proceedings of the National Academy of Sciences of the United States of America is: National Acad Sciences, 2101 Constitution Avenue NW, Washington, DC 20418, USA. Keywords: United States, West Lafayette, Life Sciences, Purdue University. This article was prepared by Science Letter editors from staff and other reports. Copyright 2007, Science Letter via NewsRx.com.
|
