Research on robotics reported by D. Tewari et al
2007 NOV 13 -- Data detailed in 'Automated extraction of avian influenza virus for rapid detection using real-time RT-PCR' have been presented. According to recent research from the United States, "Highly pathogenic H5N1 avian influenza (AI) poses a grave risk to human health. An important aspect of influenza control is rapid diagnosis This study describes the efficiency of AI-RNA extraction utilizing silica-based magnetic beads with robotics and its detection with an influenza A matrix gene real-time RT-PCR from tracheal swabs, and compares it to virus isolation and manual spin column extractions Analytical sensitivity was assessed by performing dilution analysis and detection of H2N2 AI viral RNA." "Diagnostic sensitivity and specificity was assessed by analyzing tracheal swabs collected from H7N2 infected and uninfected chickens Both manual and robotic extractions detected AI virus at 1log(10)EID(50)/ml. Diagnostic sensitivity and specificity of matrix gene detection with the automated extraction method for chicken tracheal swab specimens was similar to that of virus isolation and the manual extraction method. There were only three discordant results among 212 tested specimens The main advantages of automated robotic viral nucleic acid extraction are high throughput processing; hands-free operation; and reduction in human and technical error," wrote D. Tewari and colleagues, . The researchers concluded: "This study demonstrates successful detection of influenza A virus with magnetic beads utilizing the Qiagen MagAttract cell kit on a BioRobot M48 platform." Tewari and colleagues published their study in the Journal of Clinical Virology (Automated extraction of avian influenza virus for rapid detection using real-time RT-PCR. Journal of Clinical Virology, 2007;40(2):142-5). For additional information, contact D. Tewari, Microbiology Services, Pennsylvania Veterinary Laboratory, Harrisburg, PA 17110 USA.. Publisher contact information for the Journal of Clinical Virology is: Elsevier Science BV, PO Box 211, 1000 AE Amsterdam, Netherlands. Keywords: United States, Harrisburg, Avian Flu, Avian Influenza, Avian Influenza Virus, Bird Flu, Robotics, Viral Research, Virology. This article was prepared by Health Risk Factor Week editors from staff and other reports. Copyright 2007, Health Risk Factor Week via NewsRx.com.
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