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Data on bioengineering discussed by researchers at Osaka University
2009 AUG 5 - (NewsRx.com) -- According to recent research from Osaka, Japan, "The present study describes the morphological assessment of chondrogenic potency during a cell expanding process through serial subculturing of rabbit chondrocytes at different levels of population doublings; (PD) in a T-flask with a conventional polystyrene surface. The passaged populations were seeded on a high-density collagen surface (CL surface) and in a collagen gel (CL gel) scaffold to evaluate the planar and spatial morphologies of the chondrocytes, respectively, as well as the gene expressions of mRNA for collagen types I and II." "The planar morphological estimation was based on roundness (R-c) of chondrocyte cells at different PD values after I day incubation on the CL surface. The frequency of round-shaped cells with R-c > 0.9 (f®) decreased with increasing PD values, accompanied by an increase in collagen type I mRNA level. At PD = 17.8, the frequency reached f® = 0.12, which was less than one-sixth of that at PD = 0. A similar trend was found with respect to the passaged chondrocytes embedded in the CL gels by estimating the spatial morphology in terms of sphericity (S-c) determined 4 days after seeding," wrote M. Kinooka and colleagues, Osaka University. The researchers concluded: "With an increase in PD value, the frequency in spherical-shaped cells with S-c > 0.9 (fs) decreased and the mRNA expression of collagen type I increased, giving f(s) = 0.28 at PD = 17.8 which was less than a quarter of that at PD = 0. From these results, the cell morphologies on the CL surface and in the CL gel were proposed as indicators for understanding chondrogenic potentials concerning the phenotypes and differentiated states in the population during cell expansion, ultimately leading to quality control of tissue-engineered cartilage." Kinooka and colleagues published their study in the Journal of Bioscience and Bioengineering (Morphological evaluation of chondrogenic potency in passaged cell populations. Journal of Bioscience and Bioengineering, 2009;107(5):544-551). For additional information, contact M. Taya, Osaka University, Graduate School Engineering Science, Division Chemical Engineering, 1-3 Machikaneyama Cho, Osaka 5608531, Japan. Publisher contact information for the Journal of Bioscience and Bioengineering is: Society Bioscience Bioengineering Japan, Osaka University, Faculty Engineering, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. Keywords: Japan, Osaka, Life Sciences, Biomedicine, Bioengineering, Biomedical Engineering, Tissue Engineering, Biotechnology, Medical Device, Osaka University. This article was prepared by Biotech Week editors from staff and other reports. Copyright 2009, Biotech Week via NewsRx.com.
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