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Reports outline macromolecular research study findings from University of Minho
2009 JUL 14 - (NewsRx.com) -- "One of the goals of protein engineering is to tailor the structure of enzymes to optimize industrial bioprocesses. In the present work, we present the construction of a novel high molecular weight subtilisin, based on the fusion of the DNA sequences coding for Bacillus subtilis prosubtilisin E and for an elastin-like polymer (ELP)," investigators in Braga, Portugal report. "The resulting fusion protein was biologically produced in Escherichia coli, purified and used for wool finishing assays. When compared to the commercial protease Esperase, the recombinant subtilisinE-VPAVG(220) activity was restricted to the cuticle of wool, allowing a significant reduction of pilling, weight loss and tensile strength loss of wool fibers. Here we report, for the first time, the microbial production of a functionalized high molecular weight protease for controlled enzymatic hydrolysis of wool surface," wrote R. Araujo and colleagues, University of Minho. The researchers concluded: "This original process overcomes the unrestrained diffusion and extended fiber damage which are the major obstacles for the use of proteases for wool finishing applications.." Araujo and colleagues published their study in Biomacromolecules (Proteolytic Enzyme Engineering: A Tool for Wool. Biomacromolecules, 2009;10(6):1655-1661). For additional information, contact M. Casal, University of Minho, Dept. of Biology, CBMA Center Molecular & Environmental Biology, Campus Gualtar, P-4710057 Braga, Portugal. The publisher of the journal Biomacromolecules can be contacted at: American Chemical Society, 1155 16th St., NW, Washington, DC 20036, USA. Keywords: Portugal, Braga, Life Sciences, Biotechnology, Protein Engineering, Proteomics, Medical Device, Proteomics. Fusion Proteins, Escherichia coli, DNA Research, DNA Sequence Proteomics, Deoxyribonucleic Acid, Enzymology, Protease, Biomacromolecules, University of Minho. This article was prepared by Science Letter editors from staff and other reports. Copyright 2009, Science Letter via NewsRx.com.
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