Biotechnology

Zhejiang Gongshang University details research in gene therapy

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"The sod gene sequence was cloned into the E. coli periplasmic expression vector pBAD/gIIIA, yielding pBAD-1. E. coli was transformed using the constructed plasmid pBAD-1 and induced by adding 0.02% L-arabinose to express Cu,ZnSOD protein. The results indicated that Cu,ZnSOD enzyme activity in the periplasmic space was about fivefold to sixfold higher in the recombinant E. coli strains bearing the sod gene than in the control strains. The yields of Cu,ZnSOD were about threefold higher at 48 h than at 24 h in the recombinant E. coli cells," wrote P. Yu and colleagues, Zhejiang Gongshang University.

The researchers concluded: "Significantly higher survival of strains was obtained in cells bearing the sod gene than in the control cells when the cells were treated by heat shock and superoxide-generating agents, such as paraquat and menadione."

Yu and colleagues published their study in Applied Microbiology and Biotechnology (Enhancing survival of Escherichia coli by increasing the periplasmic expression of Cu,Zn superoxide dismutase from Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 2007;76(4):867-871).

For additional information, contact P. Yu, Zhejiang Gongshang University, College Food Science, Hangzhou 310035, People's Republic of China.

Publisher contact information for the journal Applied Microbiology and Biotechnology is: Springer, 233 Spring Street, New York, NY 10013, USA.

Keywords: People's Republic of China, Hangzhou, Biotechnology, Gene Therapy, Genetics, Genomics, Zhejiang Gongshang University.

This article was prepared by Genetics & Environmental Business Week editors from staff and other reports. Copyright 2007, Genetics & Environmental Business Week via NewsRx.com.