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Recent findings from University of Missouri highlight research in arthritis
2009 OCT 5 - (NewsRx.com) -- According to a study from the United States, "Eicosanoids and other bioactive lipid mediators are indispensable regulators of biological processes, as demonstrated by the numerous inflammatory diseases resulting from their dysregulation, including cancer, hyperalgesia, atherosclerosis, and arthritis. Despite their importance, a robust strategy comparable with gene or protein array technology for comprehensively analyzing the eicosanoid metabolome has not been forthcoming." "We have developed liquid chromatography-tandem mass spectrometry methodology that quantitatively and comprehensively analyzes the eicosanoid metabolome and utilized this approach to characterize eicosanoid production during experimental Lyme arthritis in mice infected with the bacterium Borrelia burgdorferi. Eicosanoids were extracted throughout infection from the joints of Lyme arthritis-resistant and -susceptible mice and subjected to lipidomic profiling. We identified temporal and quantitative differences between these mouse strains in the production of eicosanoids, which correlated with differences in arthritis development. The eicosanoid biosynthetic enzyme cyclooxygenase (COX)-2 has been implicated in the regulation of Lyme arthritis pathology, and subsequent lipidomic profiling of B. burgdorferi-infected COX-2(-/-) mice identified reductions not only in COX-2 products but, surprisingly, also significant off-target reductions in 5-lipoxygenase metabolites," wrote V.A. Blaho and colleagues, University of Missouri. The researchers concluded: "Our results demonstrate the utility of a comprehensive lipidomic approach for identifying potential contributors to disease pathology and may facilitate the development of more precisely targeted treatment strategies." Blaho and colleagues published the results of their research in the Journal of Biological Chemistry (Lipidomic Analysis of Dynamic Eicosanoid Responses during the Induction and Resolution of Lyme Arthritis. Journal of Biological Chemistry, 2009;284(32):21599-21612). For additional information, contact C.R. Brown, University of Missouri, Dept. of Vet. Pathobiol, 315 Connaway Hall, Columbia, MO 65211, USA. The publisher of the Journal of Biological Chemistry can be contacted at: American Society Biochemistry Molecular Biology Inc., 9650 Rockville Pike, Bethesda, MD 20814-3996, USA. Keywords: United States, Columbia, Arthritis, Atherosclerosis, Biological Chemistry, Biotechnology, Borrelia, Borreliosis, Cancer, Diagnosis, Diagnostics, Genomics, Hyperalgesia, Immunology, Lipid Research, Liquid Chromatography, Mass Spectrometry, Oncology, Protein Arrays, Proteomics, University of Missouri. This article was prepared by Pain & Central Nervous System Week editors from staff and other reports. Copyright 2009, Pain & Central Nervous System Week via NewsRx.com.
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