Researchers from Institute of Neuroscience report recent findings in brain cancer
2007 NOV 12 -- Investigators publish new data in the report 'Quantifying the A1AR distribution in peritumoural zones around experimental F98 and C6 rat brain tumours.' According to a study from Germany, "Quantification of growth in experimental F98 and C6 rat brain tumours was performed on 51 rat brains, 17 of which have been further assessed by 3D tumor reconstruction. Brains were cryosliced and radio-labelled with a ligand of the peripheral type benzodiazepine-receptor (pBR), (3)H-Pk11195 [(1-(2-chlorophenyl)-N-methyl-N-(1-methyl-propylene)-3-isoquinoline-carboxamide)] by receptor autoradiography." "Manually segmented and automatically registered tumours have been 3D-reconstructed for volumetric comparison on the basis of (3)H-Pk11195-based tumor recognition. Furthermore automatically computed areas of -300 microm inner (marginal) zone as well as 300 microm and 600 microm outer tumor space were quantified. These three different regions were transferred onto other adjacent slices that had been labelled by receptor autoradiography with the A(1) Adenosine receptor (A(1)AR)-ligand (3)H-CPFPX ((3)H-8-cyclopentyl-3-(3-fluorpropyl)-1-propylxanthine) for quantitative assessment of A(1)AR in the three different tumor zones. Hence, a method is described for quantifying various receptor protein systems in the tumor as well as in the marginal invasive zones around experimentally implanted rat brain tumours and their representation in the tumor microenvironment as well as in 3D space. Furthermore, a tool for automatically reading out radio-labelled rat brain slices from auto radiographic films was developed, reconstructed into a consistent 3D-tumour model and the zones around the tumor were visualized. A(1)AR expression was found to depend upon the tumor volume in C6 animals, but is independent on the time of tumor development," wrote M. Dehnhardt and colleagues, Institute of Neuroscience. The researchers concluded: "In F98 animals, a significant increase in A(1)AR receptor protein was found in the Peritumoural zone as a function of time of tumor development and tumor volume." Dehnhardt and colleagues published the results of their research in the Journal of Neuro-Oncology (Quantifying the A1AR distribution in peritumoural zones around experimental F98 and C6 rat brain tumours. Journal of Neuro-Oncology, 2007;85(1):49-63). For additional information, contact M. Dehnhardt, Institute of Neuroscience and Biophysics 3-Medicine, Research Centre Juelich, 52425 Juelich, Germany. The publisher of the Journal of Neuro-Oncology can be contacted at: Kluwer Academic Publ, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. Keywords: Germany, Brain Cancer, Brain Carcinoma, Neuro-Oncology, Oncology. This article was prepared by Pain & Central Nervous System Week editors from staff and other reports. Copyright 2007, Pain & Central Nervous System Week via NewsRx.com.
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