Research from University of Guelph yields new findings on monoclonal antibodies
2007 NOV 21 -- "The production of a recombinant human IgG1 in transgenic tobacco was examined to determine whether a plant-derived antibody could recruit immune system effector function against a bacterial pathogen. A plant transformation vector was engineered to contain genes for a human kappa light chain and a human gamma-1 heavy chain with V, and V, sequences from a previously identified human IgG2 monoclonal antibody (MAb) that specifically binds to and opsonizes Pseudomonas aeruginosa serotype O6ad," scientists in Guelph, Canada report. "Unique NcoI and NotI restriction sites were incorporated to flank these variable sequences, resulting in a plant transformation vector that could be engineered for expression of any other human IgG1 antibody, requiring only the substitution of other V, and V, antigen-binding coding sequences. The plant-produced IgG1 was determined to have high-mannose glycan content and to be capable of mediating opsonophagocytosis of P. aeruginosa serotype O6ad in vitro using human complement and human polymorphonuclear leukocytes," wrote M.D. Mclean and colleagues, University of Guelph. The researchers concluded: "Thus, NlAbs produced in plants from this vector could provide human IgG1 MAbs for targeting other pathogens that require the recruitment of immune system effector functions." Mclean and colleagues published their study in Antimicrobial Agents and Chemotherapy (A human anti-Pseudomonas aeruginosa serotype O6ad immunoglobulin G1 expressed in Transgenic tobacco is capable of recruiting immune system effector function in vitro. Antimicrobial Agents and Chemotherapy, 2007;51(9):3322-3328). For additional information, contact J.C. Hall, University of Guelph, Dept. of Environmental Biology, Guelph, ON N1G 2W1, Canada. The publisher's contact information for the journal Antimicrobial Agents and Chemotherapy is: American Society Microbiology, 1752 N St. NW, Washington, DC 20036-2904, USA. Keywords: Canada, Guelph, Monoclonal Antibodies, Biotechnology, Monoclonal Antibody, University of Guelph. This article was prepared by Biotech Week editors from staff and other reports. Copyright 2007, Biotech Week via NewsRx.com.
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