Study results from Medical University of South Carolina, Department of Neurosciences broaden understanding of glioblastoma therapy
2007 NOV 19 -- New investigation results, 'Methylprednisolone and indomethacin inhibit oxidative stress mediated apoptosis in rat C6 glioblastoma cells,' are detailed in a study published in Neurochemical Research. According to a study from the United States, "Glioblastoma patients receive anti-inflammatory agent for alleviation of vasogenic edema and pain prior to surgery, radiotherapy, and chemotherapy. Oxidative stress is an important mechanism of action of some chemotherapeutic agents in the treatment of glioblastoma." "So, we examined the modulatory effects of methylprednisolone (MP, a steroidal anti-inflammatory agent) and indomethacin (IM, a non-steroidal anti-inflammatory agent) on apoptosis in rat C6 glioblastoma cells following oxidative stress with hydrogen peroxide (H(2)O(2)). Exposure of C6 cells to 1 mM H(2)O(2) for 24 h caused significant amounts of morphological and biochemical features of apoptosis. Expressions of Bax and Bcl-2 at mRNA and protein levels were altered resulting in an increase in Bax : Bcl-2 ratio in apoptotic cells, which also exhibited overexpression of 80 kDa calpain and an increase in calpain-cleaved 145 kDa alpha-spectrin breakdown product. Immunofluorescent and propidium iodide labeling detected caspase-3-p20 fragment in apoptotic cells, indicating activation of caspase-3 as well. Treatment of cells with 1 microM MP or 10 microM IM alone did not induce apoptosis. Pretreatment (1 h) with either 1 microM MP or 10 microM IM significantly inhibited H(2)O(2) mediated apoptosis in C6 cells," wrote A. Das and colleagues, Medical University of South Carolina, Department of Neurosciences. The researchers concluded: "Thus, pretreatment of glioblastoma with an anti-inflammatory agent, either steroidal or non-steroidal, may compromise the action of a chemotherapeutic agent that mediates therapeutic action via oxidative stress." Das and colleagues published the results of their research in Neurochemical Research (Methylprednisolone and indomethacin inhibit oxidative stress mediated apoptosis in rat C6 glioblastoma cells. Neurochemical Research, 2007;32(11):1849-56). For additional information, contact A. Das, Medical University of South Carolina (MUSC), Dept. of Neurosciences, 96 Jonathan Lucas Street, Suite 323K, PO Box 250606, Charleston, SC 29425 USA.. The publisher of the journal Neurochemical Research can be contacted at: Kluwer Academic, Plenum Publ, 233 Spring St., New York, NY 10013, USA. Keywords: United States, Charleston, Glioblastoma Therapy, Antiinflammatory, Apoptosis, Drugs, Glioblastoma, Indomethacin, Methylprednisolone, Neurochemical Research, Oncology, Pharmaceuticals, Therapy, Treatment. This article was prepared by Biotech Business Week editors from staff and other reports. Copyright 2007, Biotech Business Week via NewsRx.com.
|
