Studies from University of Tokyo reveal new findings on colon cancer
2009 AUG 3 - (NewsRx.com) -- In this recently published study, investigators in Tokyo, Japan conducted a study "To better understand microRNA miR-21 function in carcinogenesis, we analyzed miR-21 expression patterns in different stages of colorectal cancer development using in situ hybridization (ISH). Locked nucleic acid (LNA)/DNA probes and a biotin-free tyramide signal amplification system were used in ISH analyses of miRNA expression." "Conditions for specific detection of miR-21 were determined using human cell lines and miR-21-expressing lentiviral vectors. Expression was determined in 39 surgically excised colorectal tumors and 34 endoscopically resected colorectal polyps. In the surgical samples, miR-21 expression was much higher in colorectal cancers than in normal mucosa. Strong miR-21 expression was also observed in cancer-associated stromal fibroblasts, suggesting miR-21 induction by cancer-secreted cytokines. Protein expression of PDCD4, a miR-21 target, was inversely correlated with miR-21 expression, confirming that miR-21 is indeed a negative regulator of PDCD4 in vivo. In the endoscopic samples, miR-21 expression was very high in malignant adenocarcinomas but was not elevated in nontumorigenic polyps. Precancerous adenomas also frequently showed miR-21 up-regulation. Using the LNA-ISH system for miRNA detection, miR-21 was detectable in precancerous adenomas. The frequency and extent of miR-21 expression increased during the transition from precancerous colorectal adenoma to advanced carcinoma. Expression patterns of miR-21 RNA and its target, tumor suppressor protein PDCD4, were mutually exclusive," wrote N. Yamamichi and colleagues, University of Tokyo. The researchers concluded: "This pattern may have clinical application as a biomarker for colorectal cancer development and might be emphasized by self-reinforcing regulatory systems integrated with the miR-21 gene, which has been previously shown in cell culture.." Yamamichi and colleagues published their study in Clinical Cancer Research (Locked Nucleic Acid In situ Hybridization Analysis of miR-21 Expression during Colorectal Cancer Development. Clinical Cancer Research, 2009;15(12):4009-4016). For additional information, contact H. Iba, University of Tokyo, Division Host Parasite Interact, Dept. of Microbiology & Immunology, Institute Med Science, Minato Ku, 4-6-1 Shirokanedai, Tokyo 1088639, Japan. The publisher of the journal Clinical Cancer Research can be contacted at: American Association Cancer Research, 615 Chestnut St., 17TH Floor, Philadelphia, PA 19106-4404, USA. Keywords: Japan, Tokyo, Biotechnology, Biotin, Cancer Research, Carcinogenesis, Colon Cancer, Colon Carcinoma, Colorectal, Cytokines, DNA, Dietary Supplement, Experimental Design, Gastroenterology, Gene Therapy, Micronutrient, Oncology, Polyps, Protein Expression, Proteins, Proteomics, University of Tokyo. This article was prepared by Biotech Business Week editors from staff and other reports. Copyright 2009, Biotech Business Week via NewsRx.com.
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