Research from University of Paris provide new insights into cell biology
2007 JUL 23 -- Scientists discuss in "Measurement of cystine in granulocytes using liquid chromatography-tandem mass spectrometry" new findings in cell biology. According to recent research from Paris, France, "Cystinosis is a rare autosomal recessive disorder characterized by an accumulation of intralysosomal cystine due to a defect in cystine transport across the lysosomal membrane. This disorder can be treated specifically using high doses of cysteamine." "Accurate measurement of intracellular cystine content is necessary for the diagnosis and monitoring of treatment with cysteamine. Here we describe a new method to measure intracellular cystine. It relies on a liquid chromatography-tandem mass spectrometry assay. We compare this novel method with the cystine-binding protein assay. Cells were isolated and lysed in the presence of N-ethylmaleimide to avoid interference from cysteine. After deproteinization, addition of stable isotope d6 cystine and butylation, cystine was measured using an API 3000 MSMS. The cystine assay was linear to at least 50 micromol/L. Within-run and between-run coefficients of variation were 2.9% and 5.7% respectively. It is possible to measure very low concentrations of intracellular cystine with liquid chromatography-tandem mass spectrometry," wrote A. Chabli and colleagues, University of Paris. The researchers concluded: "The results obtained with this novel method correlate very well with those obtained using the cystine-binding protein assay." Chabli and colleagues published their study in Clinical Biochemistry (Measurement of cystine in granulocytes using liquid chromatography-tandem mass spectrometry. Clinical Biochemistry, 2007;40(9-10):692-8). For additional information, contact A. Chabli, Universite Paris V, AP-HP, Hopital Necker-Enfants Malades, Service de Biochimie B, 75015 Paris, France. Publisher contact information for the journal Clinical Biochemistry is: Pergamon-Elsevier Science Ltd., the Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, England. Keywords: France, Paris, Biochemistry, Cell Biology, Cysteamine, Diagnosis, Diagnostics, Nephropathic Cystinosis Therapy, Radiation-Protective Agent. This article was prepared by Biotech Business Week editors from staff and other reports. Copyright 2007, Biotech Business Week via NewsRx.com.
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