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Studies from Catholic University add new findings in the area of cutaneous melanoma
2009 JUL 13 - (NewsRx.com) -- "Loss of heterozygosity (LOH) in several chromosomal regions is found in melanoma, and it has been partially studied in nevi. BRAF mutations are found in melanoma and nevi and in colorectal cancer are linked to mismatch repair deficiency," scientists writing in the American Journal of Dermatopathology report. "We studied early genetic events involved in melanomagenesis through analysis of allelic loss, microsatellite instability (MSI), and BRAF mutations. DNA extracted from microdissected cells of 22 common nevi, 23 atypical nevi, and 25 primary cutaneous melanomas were examined for LOH and MSI by polymerase chain reaction-based analysis of 24 microsatellite markers and BRAF mutation. Allelic loss index was higher in atypical nevi (0.20) and melanomas (0.27) than common nevi (0.07). LOFT was frequent at 9p21, 17q21, 6q231 and 5q35 in melanoma. LOH at any of this loci occurred in 27% of common nevi, 57% of atypical nevi, and 68% of melanomas. BRAF mutations were not related to MSI presence and MSI index was not related with BRAF mutational Status. Similar genetic alterations in atypical nevi and melanomas support the concept of atypical nevus as melanoma precursor. Novel deletion loci at 5q35 and 17q21 (BRCA1) in atypical nevi and melanomas were identified," wrote P. Uribe and colleagues, Catholic University. The researchers concluded: "Mismatch repair deficiency is not a crucial event for BRAF mutation in melanocytic tumors.." Uribe and colleagues published their study in American Journal of Dermatopathology (Allelotyping, Microsatellite Instability, and BRAF Mutation Analyses in Common and Atypical Melanocytic Nevi and Primary Cutaneous Melanomas. American Journal of Dermatopathology, 2009;31(4):354-363). Additional information can be obtained by contacting S. Gonzalez, Pontifical Catholic University, Dept. of Anatomy Pathology, Faculty Medical, 85 Lira St., 4th Floor, Santiago 2550681, Chile. The publisher of the American Journal of Dermatopathology can be contacted at: Lippincott Williams & Wilkins, 530 Walnut St., Philadelphia, PA 19106-3621, USA. Keywords: Chile, Santiago, Colon Cancer, Colon Carcinoma, Colorectal, Cutaneous Melanoma, DNA, Dermatopathology, Diagnosis, Diagnostics, Enzyme Research, Gastroenterology, Microsatellite Instability, Oncology, Pathology, Polymerase, Catholic University. This article was prepared by Clinical Oncology Week editors from staff and other reports. Copyright 2009, Clinical Oncology Week via NewsRx.com.
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