New research on xenotransplantion from University of Colorado summarized
2007 NOV 21 -- According to recent research published in the journal Xenotransplantation, "The isolation and testing of monkey islets after transplantation in small animal models provides basic information about their functional capacity. We describe the effect of cyclosporine A (CsA), tacrolimus (FK506) or prednisolone monotherapy on preventing monkey islet graft rejection after xenoTx in a rat model." "Histopathological aspects are reported Indian bonnet monkey (Macaca radiata radiata) islets were isolated by a simple stationary digestion technique using collagenase. The islets were purified with dextran density gradients and were transplanted under the renal capsule of normal or diabetic rats. The rats received a daily dose of CsA, or FK506, or prednisolone, and the grafts were removed at different intervals to determine islet survival. The effect of discontinuation of CsA on islet graft survival was also monitored. Histological examination of islets transplanted into normal or streptozotocin-induced diabetic rats was carried out. In diabetic rats, islet survival was determined by the graft's ability to achieve euglycemia Reversal of diabetes was achieved in all transplanted diabetic rats, demonstrating the efficacy of the isolated monkey islets. Histological examination indicated that monkey islets survived in the presence of continuous high-dose immunosuppressive monotherapy in rats. Various types of infiltrating cells were observed in the grafted area at varying times after transplantation, depending on the immunosuppressive treatment. After discontinuation of CsA, the grafts were protected for a short period This study provided evidence for monkey islet survival after transplantation into rats receiving immuno suppressive monotherapy," wrote A.N. Balamurugan and colleagues, University of Colorado. The researchers concluded: "Basic information on infiltrating cell types may be important in the study of xenograft rejection." Balamurugan and colleagues published their study in Xenotransplantation (Effect of various immunosuppressive monotherapies on survival and histopathology of monkey islet xenografts in rats. Xenotransplantation, 2007;14(4):316-322). For additional information, contact A.N. Balamurugan, University of Colorado, Health Science Center, Barbara Davis Center Childhood Diabetes, Aurora, CO, USA. The publisher's contact information for the journal Xenotransplantation is: Blackwell Publishing, 9600 Garsington Rd., Oxford OX4 2DQ, Oxon, England. Keywords: United States, Aurora, Bioengineering, Xenotransplantion, University of Colorado. This article was prepared by Biotech Week editors from staff and other reports. Copyright 2007, Biotech Week via NewsRx.com.
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