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Reports on chromatography findings from Y. Aoki and co-researchers provide new insights
2007 NOV 20 -- Fresh data on chromatography are presented in the report 'Liquid chromatography-tandem mass spectrometric method for determination of mosapride citrate in equine tissues.' According to recent research from Sagamihara, Japan, "A simple method for determination of mosapride citrate and its metabolite, des-p-fluorobenzyl mosapride (M-1), in equine muscle, liver, kidney, adipose tissue and intestine by liquid chromatography-tandem mass spectrometry has been developed. (±)-4-Amino-5-chloro-2-ethoxy-N-[ [4-(2-chlorobenzyl)morpholinyl]methyl]benzamide was used as an internal standard. The analytes and internal standard were spiked and extracted from tissues by acetonitrile." "The chromatographic separation was performed on a reversed-phase TSK-GEL SUPER ODS column with a mobile phase of acetonitrile-0.05% (v/v) formic acid containing 5 mmol/L nonafluoropentanoic acid (2:3, v/v). The method exhibited a large linear range from 0.0005 to 0.2 microg/mL for both mosapride citrate and M-1 (r >0.9976). In the intra-day assay (n=5), the relative standard deviations (RSDs) ranged from 1.1 to 7.8% for mosapride citrate and 1.6 to 7.2% for M-1. In the inter-day assay (n=3), the RSDs ranged from 1.0 to 13% for mosapride citrate and 0.8 to 11% for M-1. The extraction recovery at 1.28 microg/g of mosapride citrate from equine tissues ranged from 97 to 107%. The lower limit of quantification for mosapride citrate was found to be 0.004 microg/g. Stability studies were carried out at different storage conditions," wrote Y. Aoki and colleagues, . The researchers concluded: "The method reported is reliable, precise, and accurate and it has the capacity to be used for determination of mosapride citrate and its metabolite in tissue samples." Aoki and colleagues published their study in the Journal of Chromatography B (Liquid chromatography-tandem mass spectrometric method for determination of mosapride citrate in equine tissues. Journal of Chromatography B, 2007;858(1-2):135-42). For additional information, contact Y. Aoki, Research Institute for Animal Science in Biochemistry and Toxicology, Dept. of Analytical Chemistry, Sagamihara, Kanagawa 229-1132, Japan. Publisher contact information for the Journal of Chromatography B is: Elsevier Science BV, PO Box 211, 1000 AE Amsterdam, Netherlands. Keywords: Japan, Sagamihara, Chromatography, Diagnosis, Diagnostics. This article was prepared by Life Science Weekly editors from staff and other reports. Copyright 2007, Life Science Weekly via NewsRx.com.
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