Diagnostics

Study findings from Fukuoka University, Faculty of Pharmaceutical Sciences provide new insights into liquid chromatography

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"OPA fluorescence was achieved through an intramolecular FRET process when the molecules were excited at maximum excitation wavelength of the native fluorescent bioamines. Bioamines separated by reversed-phase LC on ODS column were derivatized with OPA and 2-mercaptoethanol," wrote M. Yoshitake and colleagues, Fukuoka University, Faculty of Pharmaceutical Sciences.

The researchers concluded: "This method provides sufficient selectivity and sensitivity for the determination of normetanephrine, dopamine, tyrosine, 5-hydroxytryptamine, tryptamine, and tryptophan in healthy human urine without prior sample purification."

Yoshitake and colleagues published their study in the Journal of Chromatography B (Liquid chromatography method for detecting native fluorescent bioamines in urine using post-column derivatization and intramolecular FRET detection. Journal of Chromatography B, 2007;858(1-2):307-12).

For additional information, contact M. Yoshitake, Faculty of Pharmaceutical Sciences, Fukuoka University, Nanakuma, Johnan, Fukuoka 814-0180, Japan.

The publisher of the Journal of Chromatography B can be contacted at: Elsevier Science BV, PO Box 211, 1000 AE Amsterdam, Netherlands.

Keywords: Japan, Fukuoka, Liquid Chromatography, Diagnosis, Diagnostics.

This article was prepared by Life Science Weekly editors from staff and other reports. Copyright 2007, Life Science Weekly via NewsRx.com.