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New stem cell research research from M.J. Dorsey and co-researchers described
2009 AUG 3 - (NewsRx.com) -- "The objective of this study is to determine if immune reconstitution of FOXP3+ T regulatory cells correlates with clinical improvement of IPEX syndrome following allogeneic hematopoietic stem cell transplant. An 8-months-old male infant with a mutation in the polyadenylation site of FOXP3 gene, absence of FOXP3 protein expression and clinical manifestations of IPEX syndrome, including eczema, colitis, failure to thrive, TPN requirement, and elevated serum IgE, underwent matched unrelated hematopoietic stem cell transplant," scientists in the United States report. "After reduced-intensity conditioning with alemtuzumab followed by fludarabine and melphalan the patient's neutrophils engrafted day +15 and platelets day +29. was a full donor chimera day +28 and +60. Intracellular FOXP3 protein expression in CD4+ T cells was absent pre-HSCT. After transplantation, percentage CD4+ T cells expressing FOXP3+CD25 bright phenotype quickly increased from 4.5 (day +29) to 23% (day +90) and continued in this trend. Foxp3 mRNA expression confirmed flow cytometry data. Serum IgE levels decreased from 5,000 IU/ml pre-transplant to 6 IU/ml on day +90, eczema resolved, and secretory diarrhea and feeding intolerance improved. T regulatory cell reconstitution is evident soon after HSCT following reduced-intensity conditioning correlating with development of full donor chimerism," wrote M.J. Dorsey and colleagues. The researchers concluded: "Increased FOXP3 expression correlates with correction of clinical and laboratory manifestations of IPEX syndrome providing direct evidence that HSCT is a curative procedure for this disorder.." Dorsey and colleagues published their study in Immunologic Research (FOXP3 expression following bone marrow transplantation for IPEX syndrome after reduced-intensity conditioning. Immunologic Research, 2009;44(1-3):179-184). For additional information, contact M.J. Dorsey, University of Southern Florida, College Medical, Division Allergy Immunology & Rheumatol, St. Petersburg, FL 33701, USA. The publisher's contact information for the journal Immunologic Research is: Humana Press Inc., 999 Riverview Drive Suite 208, Totowa, NJ 07512, USA. Keywords: United States, Alemtuzumab, Antimetabolite, Antineoplastic, Biotechnology, Bone Marrow Transplantation, Colitis, Dermatology, Drugs, Eczema, Fludarabine, Gastroenterology, Hematology, Hematopoietic, Immunodeficiency, Immunology, Immunosuppressant, Melphalan, Monoclonal Antibodies, Pharmaceuticals, Protein Expression, Proteins, Proteomics, Stem Cell Research, Surgery, Therapy, Treatment. This article was prepared by Biotech Business Week editors from staff and other reports. Copyright 2009, Biotech Business Week via NewsRx.com.
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