Research data from National University, Medical Department update understanding of liquid chromatography
2007 NOV 20 -- According to recent research published in the Journal of Lipid Research, "Here, we present an improved method for sensitive profiling of lipids in a single high-performance liquid chromatography-electrospray ionization-quadrupole time of flight mass spectrometry experiment. The approach consists of i) sensitive isocratic elution, which takes advantage of C18 column material that is resistant to increased pH values induced by piperidine, ii) chemometric alignment of mass spectra followed by differential analysis of ion intensities, and iii) semiquantitative analysis of extracted ion chromatograms of interest." "A key advantage of this method is its wide applicability to extracts that harbor lipids of considerable chemical complexity. The method allows qualitative and semiquantitative analysis of fatty acyls, glycerophospholipids (such as glycerophosphatidylinositols, glycerophosphatidylserines, and glycerophosphatidylcholines in brain extracts), phosphatidylinositol mannosides, acylated glycerophospholipids, sphingolipids (including ceramides and gangliosides in brain extracts), and, for the first time with ESI, prenols and mycolic acids (MAs). MAs are targets in antimycobacterial therapy, and they play an important immunomodulatory role during host-pathogen interactions. We compared high-resolution mass spectra of MAs derived from Mycobacterium bovis Bacille Camette-Guerin during entry into nonreplicative conditions induced by oxygen deprivation (hypoxic dormancy). Although the overall composition is not drastically altered, there are pronounced differences in individual MAs. alpha-MAs accumulate during entry into dormancy, whereas a subpopulation of keto-MAs is almost entirely eliminated. This effect is reversed upon resuscitation of dormant mycobacteria," wrote G.H. Shui and colleagues, National University, Medical Department. The researchers concluded: "These results provide detailed chemical information with relevance to drug development and immunobiology of mycobacteria." Shui and colleagues published their study in the Journal of Lipid Research (Sensitive profiling of chemically diverse bioactive lipids. Journal of Lipid Research, 2007;48(9):1976-1984). For additional information, contact M.R. Wenk, Singapore National University, Yong Loo Lin School Medical, Dept. of Biochemistry, Singapore 117597, Singapore. The publisher's contact information for the Journal of Lipid Research is: American Society Biochemistry Molecular Biology Inc., 9650 Rockville Pike, Bethesda, MD 20814-3996, USA. Keywords: Singapore, Singapore, Liquid Chromatography, Diagnosis, Diagnostics, National University, Medical Department. This article was prepared by Life Science Weekly editors from staff and other reports. Copyright 2007, Life Science Weekly via NewsRx.com.
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