Embryonal Carcinoma
Return to Library
|
Free Embryonal Carcinoma Articles |
|
|
|
|
Findings in embryonal carcinoma reported from University of Hong Kong
2009 JUN 1 - (NewsRx.com) -- According to recent research published in the journal Stem Cells, "For mouse embryonic stem (ES) cells, the importance of the S and G(2) cell cycle checkpoints for genomic integrity is increased by the absence of the G(1) checkpoint. We have investigated ionizing radiation (IR)-mediated cell cycle checkpoints in undifferentiated and retinoic acid-differentiated human embryonal carcinoma (EC) cells." "Like mouse ES cells, human EC cells did not undergo G(1) arrest after IR but displayed a prominent S-phase delay followed by a G(2)-phase delay. In contrast, although differentiated EC cells also failed to arrest at G(1)-phase after IR, they quickly exited S-phase and arrested in G(2)-phase. In differentiated EC cells, the G(2)-M-phase cyclin B1/CDC2 complex was upregulated after IR, but the G(1)-S-phase cyclin E and the cyclin E/CDK2 complex were expressed at constitutively low levels, which could be an important factor distinguishing DNA damage responses between undifferentiated and differentiated EC cells. S-phase arrest and expression of p21 could be inhibited by 7-hydroxystaurosporine, suggesting that the ataxia-telangiectasia and Rad-3-related-checkpoint kinase 1 (ATR-CHK1), and p21 pathways might play a role in the IR-mediated S-phase checkpoint in EC cells. IR-mediated phosphorylation of ataxia-telangiectasia mutated, (CHK1), and checkpoint kinase 2 were distinctly higher in undifferentiated EC cells compared with differentiated EC cells. Combined with the prominent S and G(2) checkpoints and a more efficient DNA damage repair system, these mechanisms operate together in the maintenance of genome stability for EC cells," wrote X.Q. Wang and colleagues, University of Hong Kong. The researchers concluded: "STEM CELLS 2009; 27:568-576'." Wang and colleagues published their study in Stem Cells (DNA Damage Mediated S and G(2) Checkpoints in Human Embryonal Carcinoma Cells. Stem Cells, 2009;27(3):568-576). For additional information, contact X.Q. Wang, University of Hong Kong, Dept. of Surgery, 21 Sassoon Rd., Pokfulam, Hong Kong, People's Republic of China. The publisher's contact information for the journal Stem Cells is: Alphamed Press, 318 Blackwell St., Ste. 260, Durham, NC 27701-2884, USA. Keywords: People's Republic of China, Hong Kong, DNA Damage, DNA Research, Deoxyribonucleic Acid, Embryonal Cancer, Oncology, P19 Embryonal Carcinoma, Proteomics, Retinoic Acid, Stem Cell Research, Therapy, Treatment, University of Hong Kong. This article was prepared by Clinical Oncology Week editors from staff and other reports. Copyright 2009, Clinical Oncology Week via NewsRx.com.
|
