Endometriosis

Studies from Monash University yield new information about stem cell research

"This study investigated key stem cell properties of individual clonogenic epithelial and stromal cells obtained from human endometrium. Single-cell suspensions of endometrial epithelial or stromal cells were obtained from hysterectomy tissues from 15 women experiencing normal menstrual cycles, and were cultured at clonal density ( 10 cells/cm(2)) or limiting dilution. The adult stem cell properties-self-renewal, high proliferative potential, and differentiation of single epithelial and stromal cells-were assessed by harvesting individual colonies and undertaking serial clonal culture, serial passaging, and culture in differentiation-induction media, respectively. Lineage differentiation markers were examined by RT-PCR, immunocytochemistry, and flow cytometry. Rare single human endometrial EpCAM(+) epithelial cells and EpCAM(-) stromal cells demonstrated self-renewal by serially cloning > 3 times and underwent > 30 population doublings over 4 mo in culture. Clonally derived epithelial cells differentiated into cytokeratin(+) gland-like structures in three dimensional culture. Single stromal cells were multipotent, as their progeny differentiated into smooth muscle cells, adipocytes, chondrocytes, and osteoblasts. Stromal clones expressed mesenchymal stem cell (MSC) markers ITGB1 (CD29), CD44, NT5E (CD73), THY1 (CD90), ENG (CD105), PDGFRB (CD140B), MCAM (CD146) but not endothelial or hemopoietic markers PECAM1 (CD31), CD34, PTPRC (CD45). Adult human endometrium contains rare epithelial progenitors and MSCs, likely responsible for its immense regenerative capacity, which may also have critical roles in the development of endometriosis and endometrial cancer," wrote C.E. Gargett and colleagues, Monash University.

The researchers concluded: "Human endometrium may provide a readily available source of MSCs for cell-based therapies.."

Gargett and colleagues published their study in Biology of Reproduction (Isolation and Culture of Epithelial Progenitors and Mesenchymal Stem Cells from Human Endometrium. Biology of Reproduction, 2009;80(6):1136-1145).

For additional information, contact C.E. Gargett, Monash University, Dept. of Obstetrics & Gynecology, Monash Institute Med Research, Center Women's Health Research, 246 Clayton Rd., Clayton, Vic 3168, Australia.

The publisher's contact information for the journal Biology of Reproduction is: Society Study Reproduction, 1603 Monroe St., Madison, WI 53711-2021, USA.

Keywords: Australia, Clayton, Cytometry, Gynecology, Hysterectomy, Menstruation, Progenitor Cell, Stem Cell Research, Surgery, Monash University.

This article was prepared by OBGYN & Reproduction Week editors from staff and other reports. Copyright 2009, OBGYN & Reproduction Week via NewsRx.com.