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Scientists at Sun Yat-Sen University, Department of Nephrology publish new data on angiotensins
2009 AUG 3 - (NewsRx.com) -- Researchers detail in 'Angiotensin II upregulates Toll-like receptor 4 and enhances lipopolysaccharide-induced CD40 expression in rat peritoneal mesothelial cells,' new data in angiotensins. "Activation of Toll-like receptor 4 (TLR4) in peritoneal mesothelial cells by endotoxin contributes to peritoneal inflammation and fibrosis. Here we investigated TLR4 expression induced by angiotensin II (Ang II) and functional consequences of nuclear factor-kappaB (NF-kappaB) activation and CD40 expression in rat peritoneal mesothelial cells (RPMCs)," scientists in Guangzhou, People's Republic of China report. "TLR4, CD40, tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6) were determined by reverse transcription polymerase chain reaction (RT-PCR) and TLR4, IkappaBalpha, phospho-IkappaBalpha, NF-kappaB p65, and phospho-NF-kappaB p65 were analyzed by Western blot. The intracellular distribution of NF-kappaB p65 was detected by immunofluorescence. Treatment of RPMCs with Ang II resulted in an increase in the expression of TLR4 mRNA and protein levels. Preincubation of RPMCs with Ang II significantly increased lipopolysaccharide (LPS)-induced phospho-IkappaBalpha and phospho-NF-kappaB p65 protein (p <0.05 vs. LPS alone) and CD40, TNF-alpha, and IL-6 mRNA levels (p <0.05 vs. LPS alone). A significantly increased nuclear staining of NF-kappaB p65 in cells treated with Ang II plus LPS was also observed. Ang II upregulates the expression of TLR4 by RPMCs, resulting in enhanced NF-kappaB signaling and induction of CD40, TNF-alpha, and IL-6 expression," wrote J. Wu and colleagues, Sun Yat-Sen University, Department of Nephrology. The researchers concluded: "Locally produced Ang II in the peritoneum may have an amplified role in LPS-induced peritoneal inflammation." Wu and colleagues published their study in Inflammation Research (Angiotensin II upregulates Toll-like receptor 4 and enhances lipopolysaccharide-induced CD40 expression in rat peritoneal mesothelial cells. Inflammation Research, 2009;58(8):473-82). For additional information, contact J. Wu, The First Affiliated Hospital of Sun Yat-Sen University, Dept. of Nephrology, 58 Zhongshan Road II, 510080 Guangzhou, People's Taiwan. The publisher's contact information for the journal Inflammation Research is: Springer, 233 Spring Street, New York, NY 10013, USA. Keywords: People's Republic of China, Guangzhou, Angiotensins, Angiotensin, Diagnosis, Diagnostics, Enzyme Research, Fibrosis, Hepatology, Inflammation, Necrosis, Nephrology, Polymerase. This article was prepared by Proteomics Weekly editors from staff and other reports. Copyright 2009, Proteomics Weekly via NewsRx.com.
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