Gastroenterology
Return to Library
|
Free Gastroenterology Articles |
|
|
|
|
Report summarizes parasitology study findings from F.M. Depaula and co-researchers
2009 AUG 25 - (NewsRx.com) -- "Nematode parasites from the genus Strongyloides spp. are important pathogens of the intestinal mucosa of animals and humans. Their complex life cycles involve alternating developmental adaptations between larvae stages and the adult parthenogenetic female," investigators in Uberlandia, Brazil report. "Here, we report, primarily through homology-based searching, the existence of the major components of the ubiquitin-proteasome system in this genus, using the available EST data from S. ratti, S. stercoralis, and Parastrongyloides trichosuri. In this study, S. venezuelensis was used as our model organism for detection of proteasome activity and ubiquitinated substrates in cytosolic preparations from the L3 larvae and the adult female. Marked differences in proteasome capabilities were found when these two stages were compared. A preference for degradation of chymotryptic synthetic peptides was found in both stages with the adult exhibiting a higher rate of hydrolysis compared to the larvae," wrote F.M. Depaula and colleagues. The researchers concluded: "Due to the high evolutionary conservation of proteasome alpha subunits, an anti-human proteasome antibody was able to recognize proteasome subunits in these preparations by Western blotting, supporting the proposal that the activity of the ubiqutin-proteasome system is developmentally regulated in this nematode.." Depaula and colleagues published their study in Parasitology Research (The ubiquitin-proteasome system in Strongyloididae. Biochemical evidence for developmentally regulated proteolysis in Strongyloides venezuelensis. Parasitology Research, 2009;105(2):567-576). For additional information, contact F.M. Depaula, University of Fed Uberlandia, Institute Ciencias Biomedical, Caixa Postal 593, Campus Umuarama, BR-38405302 Uberlandia, MG, Brazil. The publisher of the journal Parasitology Research can be contacted at: Springer, 233 Spring St., New York, NY 10013, USA. Keywords: Brazil, Uberlandia, Life Sciences, Gastroenterology, Parasitology, Biochemical, Parasitology Research. This article was prepared by Science Letter editors from staff and other reports. Copyright 2009, Science Letter via NewsRx.com.
|
