Studies from Southern Medical University yield new data on human papillomavirus
2009 AUG 3 - (NewsRx.com) -- "The oncoprotein E6 of high-risk human papillomavirus (HPV) types promotes cell proliferation and contributes to carcinogenesis of HPV-positive cervical cancer cells. In this study, We used small interfering RNA (siRNA) technology to silence the E6 gene in HPV-18-transformed human cervical cell line HeLa and determined the effects of E6 gene knockdown oil the cell by using microarray-based gene expression profiling coupled with gene functional classification with bioinformatics methods," researchers in Guangzhou, People's Republic of China report. "Silencing RNA prepared by siRNA expression cassettes against HPV-18 E6 gene Could significantly inhibit EO gene expression and induce HeLa cells to apoptosis. The microarray analysis identified 359 differentially expressed genes containing 307 up-regulated and 52 down-regulated genes. We analyzed the gene functions and cellular pathways in detail, including cell cycle-related genes, CCNG1 and p2l; apoptosis-related genes, CASP4, CASP6, IGFBP3, and DFFA; ubiquitin proteolysis pathway-related genes, UBE3A and UBE2C; keratinocyte differentiation-related genes, KRT4, KRT6E, and KRT18; and antioncogenes, RECK and VEL. in addition, it can be Concluded that cellular apoptosis induced by HPV-18 E6 siRNA mainly depends on the P53 and ubiquitin proteolysis pathway to regulate gene expression, consequently inhibiting cell proliferation and promoting cell apoptosis. Meanwhile, activation of antioncogene and upper regulation of immunization-related genes signified the degression of the malignant extent of tumor cells after E6 inhibition," wrote W. Min and colleagues, Southern Medical University. The researchers concluded: "Our approach, which combines the use of siRNA-mediated gene silencing, microarray screening, and functional classification of differential genes, can be used in functional genomics Study to elucidate the role of E6 oncogene in the carcinogenesis of HPV-18 and provide some possible targets for clinical treatment and drug development of cervical cancer.." Min and colleagues published their study in International Journal of Gynecological Cancer (Microarray Analysis Identifies Differentially Expressed Genes Induced by Human Papillomavirus Type 18 E6 Silencing RNA. International Journal of Gynecological Cancer, 2009;19(4):547-563). For additional information, contact W. Min, Southern Medical University, Institute Molecular Biology, Guangzhou 510515, Guangdong, People's Republic of China. Publisher contact information for the International Journal of Gynecological Cancer is: Lippincott Williams & Wilkins, 530 Walnut St., Philadelphia, PA 19106-3621, USA. Keywords: People's Republic of China, Guangzhou, Apoptosis, BioInformatics LLC, Biotechnology, Biotechnology Business, Biotechnology Company, Carcinogenesis, Cell Proliferation, Cervical Cancer, Cervical Carcinoma, Gene Therapy, Gynecology, HPV, Human Papillomavirus, Oncology, Women's Health, Southern Medical University. This article was prepared by Biotech Business Week editors from staff and other reports. Copyright 2009, Biotech Business Week via NewsRx.com.
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