Research findings from Medical College of Georgia, Medical College update understanding of cervical cancer
2009 AUG 3 - (NewsRx.com) -- "Cervical cancer originates with human papillomavirus (HPV) infection and progresses via histologically defined premalignant stages. Here we compare normal cervical epithelium and patient-matched high-grade squamous intraepithelial lesions (HSIL) with cervical carcinoma tissue from the same patient population (n = 10 per group)," scientists writing in the journal Proteomics Clinical Applications report. "Specimens were analyzed by combined laser capture microdissection and 2-D DIGE. Significant expression changes were seen with 53 spots resulting in identification of 23 unique proteins at the molecular level. These include eight that uniquely distinguish normal epithelium and HSIL and four that uniquely distinguish HSIL and carcinoma. In addition, one protein, cornulin, distinguishes all three states. Other identified proteins included differentiation markers, oncogene DJ-1, serpins, stress and interferon-responsive proteins, detoxifying enzymes, and serum transporters. A literature review, performed for all identified proteins, allowed most changes to be assigned to one of three causes: direct or indirect HPV oncoprotein interactions, growth selection during latency, or interactions in the lesion microenvironment. Selected findings were confirmed by immunohistochemistry using either frozen sections from the same cohort or formalin fixed paraffin embedded samples from a tissue microarray," wrote H. Arnouk and colleagues, Medical College of Georgia, Medical College. The researchers concluded: "Novel markers described here have potential applications for increasing the predictive value of current screening methods.." Arnouk and colleagues published their study in Proteomics Clinical Applications (Characterization of molecular markers indicative of cervical cancer progression. Proteomics Clinical Applications, 2009;3(5):516-527). Additional information can be obtained by contacting W.S. Dynan, Medical College of Georgia, Institute Molecular Med & Genetics, CB 2803, 1120 15th St., Augusta, GA 30907, USA. The publisher of the journal Proteomics Clinical Applications can be contacted at: Wiley-V C H Verlag GmbH, PO Box 10 11 61, D-69451 Weinheim, Germany. Keywords: United States, Augusta, Biotechnology, Cervical Cancer, Cervical Carcinoma, HPV, Human Papillomavirus, Oncology, Proteomics, Women's Health, Medical College of Georgia, Medical College. This article was prepared by Clinical Oncology Week editors from staff and other reports. Copyright 2009, Clinical Oncology Week via NewsRx.com.
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