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Data on immunization described by J. Liautard et al
2007 NOV 20 -- New research, 'Identification and isolation of Brucella suis virulence genes involved in resistance to the human innate immune system,' is the subject of a report. "Brucella strains are facultative intracellular pathogens that induce chronic diseases in humans and animals. This observation implies that Brucella subverts innate and specific immune responses of the host to develop its full virulence," investigators in Montpellier, France report. "Deciphering the genes involved in the subversion of the immune system is of primary importance for understanding the virulence of the bacteria, for understanding the pathogenic consequences of infection, and for designing an efficient vaccine. We have developed an in vitro system involving human macrophages infected by Brucella suis and activated syngeneic gamma9delta2 T lymphocytes. Under these conditions, multiplication of B. suis inside macrophages is only slightly reduced. To identify the genes responsible for this reduced sensitivity, we screened a library of 2,000 clones of transposon-mutated B. suis. For rapid and quantitative analysis of the multiplication of the bacteria, we describe a simple method based on Alamar blue reduction, which is compatible with screening a large library. By comparing multiplication inside macrophages alone and multiplication inside macrophages with activated gamma9delta2 T cells, we identified four genes of B. suis that were necessary to resist to the action of the gamma9delta2 T cells," wrote J. Liautard and colleagues, . The researchers concluded: "The putative functions of these genes are discussed in order to propose possible explanations for understanding their exact role in the subversion of innate immunity." Liautard and colleagues published their study in Infection and Immunity (Identification and isolation of Brucella suis virulence genes involved in resistance to the human innate immune system. Infection and Immunity, 2007;75(11):5167-74). For additional information, contact J. Liautard, CNRS-UMR 5236 and Universite Montpellier II, CC100, place Eugene Bataillon, 34000 Montpellier, France. The publisher of the journal Infection and Immunity can be contacted at: American Society Microbiology, 1752 N St. NW, Washington, DC 20036-2904, USA. Keywords: France, Montpellier, Immunization, Immunology. This article was prepared by Life Science Weekly editors from staff and other reports. Copyright 2007, Life Science Weekly via NewsRx.com.
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