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Reports outline gene therapy research from University of Pittsburgh, Medical Department
2009 JUL 20 - (NewsRx.com) -- According to a study from the United States, "With the completion of genome sequences of major model organisms, increasingly sophisticated genetic tools are necessary for investigating the complex and coordinated functions of genes. Here we describe a genetic manipulation system termed ''genomic engineering'' in Drosophila." "Genomic engineering is a 2-step process that combines the ends-out (replacement) gene targeting with phage integrase phi C31-mediated DNA integration. First, through an improved and modified gene targeting method, a founder knockout line is generated by deleting the target gene and replacing it with an integration site of phi C31. Second, DNA integration by phi C31 is used to reintroduce modified target-gene DNA into the native locus in the founder knock-out line. Genomic engineering permits directed and highly efficient modifications of a chosen genomic locus into virtually any desired mutant allele," wrote J. Huang and colleagues, University of Pittsburgh, Medical Department. The researchers concluded: "We have successfully applied the genomic engineering scheme on 6 different genes and have generated at their loci more than 70 unique alleles." Huang and colleagues published the results of their research in Proceedings of the National Academy of Sciences of the United States of America (Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering. Proceedings of the National Academy of Sciences of the United States of America, 2009;106(20):8284-8289). For additional information, contact Y. Hong, University of Pittsburgh, School Medical, Dept. of Cell Biology & Physiol, Pittsburgh, PA 15261, USA. The publisher of the journal Proceedings of the National Academy of Sciences of the United States of America can be contacted at: National Acad Sciences, 2101 Constitution Avenue NW, Washington, DC 20418, USA. Keywords: United States, Pittsburgh, Biotechnology, DNA, Enzymology, Gene Therapy, Genetics, Genomics, Integrase, University of Pittsburgh, Medical Department. This article was prepared by Proteomics Weekly editors from staff and other reports. Copyright 2009, Proteomics Weekly via NewsRx.com.
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