New findings from Kaohsiung Medical University, Institute of Pharmaceutical Science describe advances in chromatography
2007 AUG 28 -- Fresh data on chromatography are presented in the report "A sensitive liquid chromatographic method for the analysis of isovaleric and valeric acids in urine as fluorescent derivatives." According to recent research published in the Journal of Chromatography A, "A simple and sensitive isocratic liquid chromatographic method was developed for the analysis of isovaleric and valeric acids in human urine as biomarkers in metabolic acidosis. The method is based on the derivatization of isovaleric and valeric acids with a fluorescent reagent 2-(2-naphthoxy)ethyl-2-(piperidino)ethanesulfonate for labeling the analytes with the naphthoxy fluorophore." "The resulting fluorescent derivatives of isovaleric and valeric acids were separated on a phenyl-hexyl column, using a mixed solvent of methanol-water-tetrahydrofuran (55:31:14, v/v) as the mobile phase. The separated derivatives were monitored with a fluorimetric detector (excitation at 225 nm and emission at 360 nm). The linear range of the method for the determination of isovaleric acid or valeric acid derivative was over 0.2 approximately 8.0 microM. The detection limit (signal to noise ratio=3 with 10 microl injected) of isovaleric acid or valeric acid was about 0.04 microM," wrote T.C. Chung and colleagues, Kaohsiung Medical University, Institute of Pharmaceutical Science. The researchers concluded: "Application of the method to the analysis of isovaleric acid in the urine of a patient with isovaleric acidemia proved feasible." Chung and colleagues published their study in the Journal of Chromatography A (A sensitive liquid chromatographic method for the analysis of isovaleric and valeric acids in urine as fluorescent derivatives. Journal of Chromatography A, 2007;1156(1-2):259-63). For additional information, contact T.C. Chung, Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan. The publisher's contact information for the Journal of Chromatography A is: Elsevier Science BV, PO Box 211, 1000 AE Amsterdam, Netherlands. Keywords: Taiwan, Kaohsiung, Chromatography. This article was prepared by Life Science Weekly editors from staff and other reports. Copyright 2007, Life Science Weekly via NewsRx.com.
|
